货品编号： NEB .R3539S

品牌：NEB

品名： BbsI-HF®

规格： 300 units

Unit Definition One unit is defined as the amount of enzyme required to digest 1 µg of λDNA in 1 hour at 37°C in a total reaction volume of 50µl. Reaction Conditions 1X CutSmart® Buffer Incubate at 37°C 1XCutSmart® Buffer: 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 100 μg/ml BSA pH 7.9 @ 25°C Activity in NEBuffers NEBuffer 1.1: 10% NEBuffer 2.1: 10% NEBuffer 3.1: 10% CutSmart® Buffer: 100%Diluent Compatibility Diluent B Storage Temperature -20°C Storage Conditions 10 mM Tris-HCl 300 mM NaCl 1 mM DTT 0.1 mM EDTA 200 μg/ml BSA 50% Glycerol pH 7.4 @ 25°C Heat Inactivation65°C for 20 min Methylation Sensitivity dammethylation: Not Sensitive dcmmethylation: Not Sensitive CpG Methylation: Not Sensitive