货品编号： Thermo.47-9200-42 品 牌：Thermo 品 名：Invitrogen CD200 Monoclonal Antibody (OX104), APC-eFluor 780, eBioscience 规 格： 100tests 产品描述： Description: The monoclonal antibody OX104 recognizes human CD200 also known as OX2. CD200 is a member of the Ig superfamily

with 2 Ig domains, a transmembrane and cytoplasmic domain. CD200 is expressed on resting and activated B cells, a subset of resting

and activated T cells, keratinocytes, peripheral and central nerve cells, follicular dendritic cells and ovarian cells. The interaction with

CD200R results in macrophage activation (IL-6 production), inhibition of mast cell degranulation along with reduced TNF alpha and IL-13

secretion and overall attenuation of the activation status of lymphocytes. A role has also been suggested in maternal tolerance as

expression of CD200 is also present on the trophoblast.

Applications Reported: This OX104 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OX104 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood

cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final

volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633-647 nm). Please make sure that your instrument is capable of

detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step

Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.

Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser