货品编号： Thermo.A10044 品 牌：Thermo 品 名：Invitrogen EdU (5-ethynyl-2-deoxyuridine) 规 格： 50mg 产品描述： EdU (5-ethynyl-2’-deoxyuridine) can be used as a replacement for BrdU (5-bromo-2’-deoxyuridine) and directly

measures de novo DNA synthesis or S-phase synthesis of the cell cycle using click chemistry. Click chemistry is

a method of covalently coupling an azide with an alkyne. Detection of EdU employs the copper(I) catalyzed click

reaction with an azide modified fluorescent dye to form a stable triazole ring. Because of the small size of the click

detection reagent, no harsh denaturation steps are needed to gain access to the DNA. Eliminating this step allows

for more reproducible results, a simpler and quicker protocol and measurements which can easily be multiplexed

with relevant antibody based targets including phospho-histone H3, Ki-67, and cyclin B1 as well as dual-pulse

experiments with BrdU by flow cytometry, fluorescence microscopy, microplate (HTS) or high-throughput imaging (HCS).

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.