货品编号： ebioscience.16-7311-85 品 牌：ebioscience 品 名：IFN gamma Monoclonal Antibody (XMG1.2), Functional Grade 规 格： 500ug 产品描述： Description: The XMG1.2 antibody reacts with mouse interferon (IFN) gamma. The XMG1.2 antibody is a neutralizing antibody.

Mouse IFN gamma is a 20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine.

IFN gamma, in synergy with other cytokines such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory

effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN

gamma does not affect its biological activity.

Applications Tested: The Functional Grade Purified XMG1.2 antibody has been tested by LAL assay to verify low endotoxin levels and has

been tested for ELISA capture and in bioassay for neutralization of IFN gamma bioactivity.

The XMG1.2 antibody at 15 ng/mL has been found to inhibit by 50% the biological effects of 1 ng/mL of mouse IFN gamma (ND50), in an

EMCV assay of L929 cell protection. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using

antibodies can be found in the BestProtocols® section.

The XMG1.2 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IFN gamma in combination with

the biotin R4-6A2 (13-7312) antibody for detection and recombinant mouse IFN gamma (39-8311) as the standard. A suitable range of

concentrations of this antibody for ELISA capture is 0.25 - 1 µg/mL. A standard curve consisting of doubling dilutions of the recombinant

standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.