货品编号： abcam.ab176757

品牌： abcam

品名： Phalloidin-iFluor 594 Reagent

规格： 300T

• Product namePhalloidin-iFluor 594 ReagentSee all F-actin kits

• Sample typeAdherent cells, Suspension cells
• Assay typeCell-based (qualitative)
• Product overview

  Phalloidin-iFluor 594 Reagent (ab176757) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phalloidin-Fluor 594 can be detected with a fluorescent microscope at Ex/Em = 590/618 nm.

  Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plantactin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.

  Review other popular phalloidin dye conjugates, includingPhalloidin-iFluor 488,Phalloidin-iFluor 647,Phalloidin-iFluor 555, andRhodamine Phalloidin, search the website to seeall phallodin conjugates, or read thephalloidin staining protocol.

• Notes

  Stainingfixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.

  When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.

• PlatformFluorescence microscope