| 研究领域 | 细胞生物神经生物学信号转导生长因子和激素转录调节因子激酶和磷酸酶细胞因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat, (predicted: Dog,Pig,Cow,Horse,Rabbit,Guinea Pig,) |
| 产品应用 | WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestICC=1:100-500IF=1:200-800（石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user. |
| 分子量 | 13/27kDa |
| 细胞定位 | 分泌型蛋白 |
| 性状 | Liquid |
| 浓度 | 1mg/ml |
| 免疫原 | KLH conjugated synthetic peptide derived from human BDNF:151-247/247 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储存液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | Neurotrophins function to regulate naturally occurring cell death of neurons during development. The prototype neurotrophin is nerve growth factor (NGF), originally discovered in the 1950s as a soluble peptide promoting the survival of, and neurite outgrowth from, sympathetic ganglia. More recently, three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4), also designated NT-5. These various neurotrophins stimulate the in vitro survival of distinct but partially overlapping populations of neurons. The Trk A receptor is the preferential receptor for NGF, but also binds NT-3 and NT-4. The Trk B receptor binds equally well to both BDNF and NT-4 and to a lesser extent NT-3, while the Trk C receptor only binds NT-3. BDNF promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Belongs to the NGF-beta family.Function:During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.Subunit:Monomers and homodimers. Binds to NTRK2/TRKB.Subcellular Location:Secreted.Tissue Specificity:Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.Post-translational modifications:The propeptide is N-glycosylated and glycosulfated.DISEASE:Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.Similarity:Belongs to the NGF-beta family.SWISS:P23560Gene ID:627Database links: Entrez Gene: 627Human Entrez Gene: 12064Mouse Entrez Gene: 24225Rat Omim: 113505Human SwissProt: P23560Human SwissProt: P21237Mouse SwissProt: P23363Rat Unigene: 502182Human Unigene: 1442Mouse Unigene: 11266Rat Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | Sample:Cerebrum (Mouse) Lysate at 40 ugCerebellum (Mouse) Lysate at 40 ugCerebrum (Rat) Lysate at 40 ugPrimary: Anti-BDNF (bs-4989R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 13/27 kDObserved band size: 18/32 kDParaformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BDNF) Polyclonal Antibody, Unconjugated (bs-4989R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BDNF) Polyclonal Antibody, Unconjugated (bs-4989R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: U251 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (BDNF) Polyclonal Antibody, Unconjugated (bs-4989R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.Tissue/cell: BV-2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (BDNF) Polyclonal Antibody, Unconjugated (bs-4989R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BDNF) Polyclonal Antibody, Unconjugated (bs-4989R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.Blank control(blue): HepG2(fixed with 2% paraformaldehyde for 10 min at 37℃).Primary Antibody:Rabbit Anti-BDNF antibody (bs-4989R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. |
QQ:1749072012