Bioss.bs-0990R Rabbit Anti-HPV16 E6 antibody 50ulBioss-耗材-金山科研平台 Thermo Fisher官网Nunc、QSP、Nalgene、Invitrogen、Gibco耗材试剂授权一级代理

Bioss.bs-0990R Rabbit Anti-HPV16 E6 antibody 50ulBioss

货品编号： Bioss.bs-0990R

品牌： Bioss

品名： Rabbit Anti-HPV16 E6 antibody

规格： 50ul

产品编号	bs-0990R
英文名称	HPV16 E6
中文名称	人类乳头状瘤病毒16抗体
别名	E6; Human Papilloma Virus; Human papillomavirus type 16 E6; Human papillomavirus type 16; Protein E6; E6 protein; HPV16 E6; HPV16-E6; VE6_HPV16.
	Specific References(2)\|bs-0990R has been referenced in 2 publications.[IF=3.65]Shao, Jian-Shuang, et al. "HPV16 E6/E7 upregulates HIF-2α and VEGF by inhibiting LKB1 in lung cancer cells." Tumor Biology 39.7 (2017): 1010428317717137.WB;Human.PubMed:28720067[IF=2.8]Yang, J. H., et al. "Long-term persistent infection of HPV 16 E6 up-regulate SP1 and hTERT by inhibiting LKB1 in lung cancer cells." PloS one 12.8 (2017): e0182775.WB;Human.PubMed:28813465
研究领域	肿瘤细菌及病毒
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human,
产品应用	ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
分子量	11kDa
性状	Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human HPV16 E6:85-158/158
亚型	IgG
纯化方法	affinity purified by Protein A
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed	PubMed
产品介绍	Human papilloma viruses (HPVs) can be classified as either high risk or low risk according to their association with cancer. HPV16 and HPV18 are the most common of the high risk group while HPV6 and HPV11 are among the low risk types. Approximately 90% of cervical cancers contain HPV DNA of the high risk types. Mutational analysis have shown that the E6 and E7 genes of the high risk HPVs are necessary and sufficient for HPV transforming function. The specific interactions of the E6 and E7 proteins with p53 and pRB, respectively, correlate with HPV high and low risk classifications. The high risk HPV E7 proteins bind to pRB with a higher affinity than do the low risk HPV proteins, and only the high risk HPV E6 proteins form detectable complexes with p53 in vitro.Function:Plays a major role in the induction and maintenance of cellular transformation. Acts mainly as an oncoprotein by stimulating the destruction of many host cell key regulatory proteins. E6 associates with host E6-AP ubiquitin-protein ligase, and inactivates tumor suppressors TP53 and TP73 by targeting them to the 26S proteasome for degradation. In turn, DNA damage and chromosomal instabilities increase and lead to cell proliferation and cancer development. The complex E6/E6P targets several other substrates to degradation via the proteasome including host NFX1-91, a repressor of human telomerase reverse transcriptase (hTERT). The resulting increased expression of hTERT prevents the shortening of telomere length leading to cell immortalization. Other cellular targets including Bak, Fas-associated death domain-containing protein (FADD) and procaspase 8, are degraded by E6/E6AP causing inhibition of apoptosis. E6 also inhibits immune response by interacting with host IRF3 and TYK2. These interactions prevent IRF3 transcriptional activities and inhibit TYK2-mediated JAK-STAT activation by interferon alpha resulting in inhibition of the interferon signaling pathway.Subunit:Forms a complex E6-AP ubiquitin-protein ligase which interacts with human TP53. Binds to human FBLN1 and MPDZ (By similarity). Interacts with human NFX1 and MAGI3. Interacts with human IRF3; this interaction inhibits the establishment of antiviral state. Interacts with human TYK2; this interaction inhibits JAK-STAT activation by interferon alpha.Subcellular Location:Host nucleus matrix.Similarity:Belongs to the papillomaviridae E6 protein family.SWISS:P03126Gene ID:1489078Database links: Entrez Gene: 1489078 HPV16 E6 Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片	Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;Incubation: Anti-HPV16-E6 Polyclonal Antibody, Unconjugated(bs-0990R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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Bioss.bs-0990R Rabbit Anti-HPV16 E6 antibody 50ulBioss

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