代理WNT-5a, Human 25µg 生长分化因子 现货


产品英文名称: WNT-5a, Human

产品中文名称:人重组WNT-5a蛋白

WNT-5a, Human产品介绍:

Wnt信号转导通路因其启动蛋白为Wnt蛋白而得名,在胚胎发育、细胞增殖及分化等方面发挥重要 作用,其异常活化与人类多种肿瘤的发生密切相关。研究显示,Wnt信号至少存在经典和非经典两条不同的信号通路,而Wnt-5a属于非经典Wnt信号通路,既往研究Ror2具有Wnt受体结构域样结构。有研究认为Wnt-5a经由Ror2介导的 信号通路发挥作用。

Human WNT-5a蛋白含有357个氨基酸残基。

WNT-5a, Human 产品特性:

» 活性在共转Frizzled- 4 和 LRP- 5基因的HEK293 细胞鉴定,纯度高,无动物成分污染

» WNT-5a, Human活性在共转Frizzled- 4 和 LRP- 5基因的HEK293 细胞鉴定

» WNT-5a, Human产品经过SDS-PAGE 和HPLC双层鉴定纯度大于85%

WNT-5a, Human产品英文简述:

WNT-5a belongs to the class of WNT proteins that activate the “noncanonical” pathway. The predicted size of human WNT-5a is a monomeric protein containing 357 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of ~45 kDa on SDS-PAGE under non-reducing conditions. StemRD’s product is expressed from a human cell line in animal-free medium, and purified with a proprietary process that is distinct from the published method.

• Purity: Greater than 85% as determined by SDS-PAGE and HPLC analysis

• Biological Activity: The activity was determined by using a TCF reporter gene assay in HEK293 cells co-transfected with Frizzled-4 and LRP- 5. WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay (MilkelsAJ, et al., PLoS Biol, 4:e115, 2006). This activation mode is utilized because activation assays are generally more reliable than inhibition assays, as they are less prone to any non-specific inhibitory contamination in the preparation.

© 金山科研平台 Thermo Fisher官网Nunc、QSP、Nalgene、Invitrogen、Gibco耗材试剂授权一级代理是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:https://thermonunc.cn/thread-18724.htm
产品询价需求提交
返回