产品英文名称：Noggin,human

产品中文名称：人Noggin蛋白

Noggin,human 产品介绍：

Noggin 被认为是同时对BMP及抗BMP生物活性物质均具有高亲和性的结合蛋白中的一种。在胚胎干细胞培养过程中加入Noggin，可以有效提高干细胞的多能性。

重组的人Noggin蛋白在进行移动单一多肽链处理后共包含216个氨基酸，并且达到预测的分子量24.6kDa。 糖基化反应后，随着反应条件的变化，Noggin逐渐集结成SDS-PAGE从而达到大约30kDa的分子量。

StemRD公司生产的Noggin来源于经过翻译后特异性修饰的人293细胞，且经过一系列的色谱分析方法对产品进行纯化。

Noggin,human产品特性：

» SDS-PAGE检测纯度大于95%

» 生物活性在人293细胞中用受体基因检测确定，EC50在25 - 100 ng/mL之间

» Noggin,human只限于科学研究，非人体使用

Noggin,human 产品英文简述：

Noggin has been shown to be a high-affinity binding protein to BMP and antagonizes BMP bio-activities. It can be added to cell culture medium for embryonic stem cells to maintain pluripotency.

The recombinant human Noggin comprises 216 amino acids after removal of the signal peptide and has a predicted molecular mass of 24.6 kDa. As aresult of glycosylation, it migrates as an approximately 30 kDa band in SDS-PAGE under reducing conditions.

StemRD's Noggin is made in human 293 cells, which renders humanspecific post-translational modifications. We purified this product through a series of chromatography.

• Purity：Greater than 95% by SDS-PAGE

• Biological Activity：The activity was determined by using a reporter gene assay in human 293 cells for the inhibition of activity of BMP4 (2.5ng/mL). The EC50 ranges from 25 - 100 ng/mL

• For Research Use Only. Not for Use in Humans