产品英文名称：Frizzled-5 Fc Fusion, human

产品中文名称：人Frizzled-5 Fc Fusion融合蛋白

Frizzled-5 Fc Fusion, human产品介绍：

Frizzled-5是WNT蛋白10个哺乳类卷曲受体之一。卷曲蛋白细胞外的半胱氨酸富集区与WNTs的密切关系可达到nM级别。当被作为重组蛋白时，Frizzled-5的细胞外区域就成为可溶的受体，以阻止细胞中WNTs与Frizzled的结合。

StemRD公司的Frizzled-5细胞外主蛋白是与人IgG1细胞Fc片段的N-终端相融合的。这种融合保证了体内外蛋白的稳定性，以及重组蛋白二聚体对WNTs的高亲和性。研究基因科技的科学家曾使用与之类似的蛋白Frizzled-8 Fc融合，证实了动物模型中WNT封锁物的抗肿瘤效果（DeAlmeida VI et al，Cancer Res.2007,67:5371）。

用无血清无蛋白的培养基培养CHO细胞，看其基因表达情况。使用蛋白A色谱分析法对特定培养基条件下的融合蛋白进行纯化。

Frizzled-5 Fc Fusion, human产品英文简述：

Frizzled-5 is one of the 10 mammalian Frizzled receptors for WNT proteins. The extracellular cycteine rich domain of Frizzleds bind to WNTs with nM affinity. When supplied as recombinant protein, this extracellular domain of Frizzled-5 acts as a soluble receptor, blocking the binding of WNTs to Frizzled on the cells.

StemRD's Frizzled-5 extracellular domain protein is fused to the Nterminus of the Fc domain of human IgG1. This fusion allows stabilization of the protein in vitro and in vivo and dimerization of the recombinant protein rendering high-affinity binding to WNTs. A similar protein, Frizzled-8 Fc fusion, has been used by Genentech scientists to show antitumor effect of WNT blockade in animal models (DeAlmeida VI et al, Cancer Res. 2007, 67:5371).

Expression is from CHO cells cultured in serum-free and protein-free medium. Purification of the fusion protein from conditioned culture medium is performed using protein A chromatography.

• Purity：Greater than 95% as determined by SDS-PAGE analysis

• Biological Activity：The activity was determined by using a TCF reporter gene assay in cultured

human cells. The IC50 ranges from 100 - 200 ng/ml in the inhibition of 100 ng/mL WNT-3a activity.