MycoAlert™ Mycoplasma Detection Kit产品介绍：

Lonza MycoAlert Mycoplasma Detection Kit支原体检测试剂盒是专门检测细胞培养过程中是否被支原体污染的经典试剂盒，它包含检测所用到的各种试剂和产品操作手册。MycoAlert™支原体检测试剂盒检测原理主要是：细胞培养中存活的支原体被溶解，然后释放出来的酶与支原体检测试剂盒中提供的底物发生催化反应，将ADP转化成ATP。通过测量样品中添加底物前后ATP的含量，可以得到一个比率，该比率指示支原体是否存在。如果这些酶不存在，第二次读数相对于第一次读数就没有增加；如果酶与底物进行特异性反应，就会导致ATP含量上升。最后通过分光光度计读数，由于发出光的强度与ATP的浓度成线性关系，因此可检测支原体的污染问题。同时，该支原体检测试剂盒具有操作简单、快速高效、结果稳定可靠、灵敏度极高等优点，让您在支原体检测时轻松容易，并确保您的培养洁净无污染。

MycoAlert™ Mycoplasma Detection Kit产品特性：

» 快速检测：20分钟内即可诊断出你的细胞培养是否健康无污染，而其它方法一般要几个小时到一周的时间

» 应用方便：只需把你的细胞培养物与两种试剂混合配成100µl，在分光光度计上读2次数

» 通用性强：可以检测所有支原体在细胞培养过程中的污染，如支原体属、无胆甾原体属、虫原体属、脲原体属、螺原体属等

» 检测灵敏：可检测<50 cfu/ml的支原体，避免出现像PCR和荧光染色方法出现的阴性结果

» 成分完备：该支原体检测试剂盒包含实验所需的所有试剂，而不像商业PCR检测方法中需要你提供PCR酶、核酸、限制性内切酶和琼脂糖电泳所需的其它试剂

MycoAlert™ Mycoplasma Detection Kit英文简述：

The MycoAlert™ Mycoplasma Detection Kit is a selective biochemical test that exploits the activity of mycoplasmal enzymes which are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells. Viable mycoplasma in a test sample (cell supernatant) are lysed and the enzymes react with the MycoAlert™ Substrate, catalyzing the conversion of ADP to ATP. The ATP is then transferred into a light signal via the luciferase enzyme in the MycoAlert™ Reagent. By measuring the level of ATP in a sample both before (read A) and after the addition of the MycoAlert™ Substrate (read B), a ratio can be obtained which is indicative of the presence or absence of mycoplasma.

Convenient & fast

• Simply add two reagents to the culture supernatant

• Perform two luminescence readings

• Easy interpretation of results

• Results in < 20 min

Universal

• Detects all common mollicute contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma; except Ureaplasma)

• Negligible interference with media components