产品英文名称：Mouse Ultrasensitive Insulin ELISA Kit

产品中文名称：鼠超灵敏胰岛素ELISA检测试剂盒

§ 检测原理：ALPCO鼠胰岛素超灵敏ELISA是一个夹层式免疫检测试剂盒。96孔微孔板涂有一层胰岛素特异性单克隆抗体。将标准物，对照品和样品一并添加到微型板小孔中与配体混合。然后将微孔板放在摇床上温育2小时，700－900转/分钟。第一次温育结束，用清洗缓冲液清洗小孔并烘干。添加TMB作用底物，放在微孔板摇床上第二次温育30分钟，700－900转/分钟。第二次温育结束，添加反应终止液，调节分光光度计到450nm测定OD值。由于发光强度与样品中胰岛素含量成比例，因此可以根据OD值确定胰岛素含量。

§ 检测过程：各添加5μl/25µl标准物，对照物和样品→加75µl配体物→微孔板摇床温育2小时，700－900rpm→清洗缓冲液清洗6次→加100µl TMB作用物→微孔板摇床温育30分钟，700－900rpm→添加100µl反应终止液→分光光度计450nm读数

Mouse Ultrasensitive Insulin ELISA Kit 英文简述：

The ALPCO Mouse Ultrasensitive Insulin ELISA kit quantifies the concentration of the insulin protein products of the mouse I and mouse II proinsulin genes from as little as 5µl of serum or plasma sample. Utilizing a dual-monoclonal antibody sandwich format, bi-level control set, and 96-well microplate comprising removable strips, a single Mouse Ultrasensitive Insulin ELISA kit has the performance characteristics and flexibility necessary to confidently measure up to 40 samples in duplicate. The shelf-life of the components and the resealable microplate pouch allow for convenient storage for future use if the entire kit is not needed at one time.

The ALPCO Mouse Ultrasensitive Insulin ELISA is provided with seven standards designed to meet the needs of either very small sample size, or very low insulin concentrations. For small samples, such as tail snip samples, use a sample size of 5µl and the top five standards, 0.18-6.9 ng/ml. For hypoinsulinemic samples from a type 1 diabetes model or streptozotocin treatment, use a 25µl sample size and the bottom five standards, 0.025-1.25 ng/ml.