产品英文名称：SeaKem® Gold Agarose

产品中文名称：SeaKem® Gold琼脂糖粉

SeaKem® Gold Agarose琼脂糖 产品介绍：

SeaKem® Gold Agarose是一种高凝胶强度，低EEO（电渗），标准凝胶温度的琼脂糖。SeaKem® Gold Agarose属于遗传技术级别，最适于在脉冲场凝胶电泳实验（PFGE）中快速分离巨大碱基对DNA。低电渗性能使得其在凝胶电泳实验中DNA电泳迁移率明显大于使用传统琼脂糖，调节缓冲液和琼脂糖浓度可以使跑胶时间减少一半（具体方法参照说明书）。高凝胶强度使其在低浓度时很容易操作，能够使用传统电泳分离大DNA片段，减少分离DNA片段时间（PFGE）。

SeaKem® Gold Agarose 产品参数：

› Gelling temperature (1.5%) ――36°C ±1.5°C

› Melting temperature (1.5%)―― >90°C

› Gel strength (1%)―― >1,800 g/cm²

› Gel strength (1.5%)―― >3,500 g/cm²

SeaKem® Gold Agarose产品应用：

• Preparative DNA and RNA electrophoresis/电泳制备DNA/RNA

• Analysis of Long PCR† reactions/长PCR反应分析

• Separation and further manipulation of DNA >1,000 bp/分离1000-50000 bp DNA

• Analytical electrophoresis of DNA and RNA /DNA和RNA分析电泳

• Blotting of DNA and RNA /DNA和RNA杂交

SeaKem® Gold Agarose 英文简述：

SeaKem® Gold Agarose is a very high gel strength, low EEO, standard gelling temperature agarose. This Genetic Technology Grade™ Agarose is for rapid resolution of megabase DNA by pulsed field gel electrophoresis (PFGE). Due to its low EEO, the electrophoretic mobility of DNA in SeaKem® Gold Agarose gels is significantly greater than in conventional agarose gels. Run times for PFGE can be decreased by as much as 50% depending upon buffer and agarose concentration. Due to its high gel strength, SeaKem® Gold Agarose forms easy-to-handle gels at low concentrations (as low as 0.5%), which allow for the separation of larger DNA fragments by conventional electrophoresis as well as a decrease in the time needed to separate DNA by PFGE. For the recovery of megabase size DNA fragments following PFGE separation, we recommend SeaPlaque™ GTG™ Agarose and ß-Agarase.

Related Products:

• AccuGENE® 10X TAE Buffer

• GelStar® Nucleic Acid Gel Stain

• Megabase DNA Standards

• InCert® Agarose