产品英文名称:Nu-Serum™ Serum Replacement
产品中文名称:Nu-Serum™ 血清替代品
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BD Nu-Serum™血清替代品 产品介绍:
NuSerum™血清替代品是一种生长培养基补体,可用于替代胎牛血清,它与用于细胞培养的小牛血清、胎牛血清和其它常用血清相比有较低蛋白含量。低蛋白成份有利于蛋白纯化、病毒生产、单克隆抗体生产和筛选,并且能提高细胞转染成功率。BD NuSerum™血清替代品组成成份:表皮生长因子,内皮细胞生长补体,胰岛素,转铁球蛋白,三碘甲状腺氨酸,孕酮,睾酮,Beta 雌二醇-17,硒,O-磷酸乙醇胺,葡萄糖,氨基酸,维生素,其它成份和25%新生牛血清。
质量控制:
• 总蛋白成份,pH,摩尔等渗透压浓度测试
• 0.2µm膜过滤检测对细菌,真菌,支原体和病毒为阴性
• 细胞病变效应(CPE),包涵体和血细胞吸附性能验证
• 内毒素检测(LAL鲎试剂检测)
BD Nu-Serum™ Serum Replacement English Description:
NuSerum™ Serum Replacements is a growth medium supplement which provides a low-protein alternative to newborn calf, fetal bovine, and other sera routinely used for cell culture. The low-protein content facilitates protein purification, virus production (Okada, M., et al., Microbiological Immunol. 31:657, 1987), monoclonal antibody production and screening (Sjogren-Janssen, E., e al., J. Immunol. Meth. 84:357, 1985), and increases the frequency of successful transfection of cells (Seed, B., et al., Proc. Natl. Acad. Sci. USA 84:3365, 1987; Duval, N., et al., J. Cell Biol. 118:641, 1992).
Quality Control:
• Tested at a concentration of 10% for the ability to stimulate a >= 100-fold increase in growth of BHK-21 and a >= 13-fold increase in growth of BALB/c-3T3 cells.
• Tested for total protein content, pH, and osmolarity (information included with product).
• Filtered (0.2 µm membrane) and tested and found negative for the presence of bacteria, fungi, mycoplasma, and viruses (bovine diarrhea virus, bovine parvovirus, bovine adenovirus, reovirus, and rabies virus); also tested for the absence of cytopathic effects (CPE), inclusion bodies and hemadsorption.
• Tested for endotoxin (LAL assay).
订购信息:
355100 Nu-Serum™ Serum Replacements, 100ml
355504 Nu-Serum™ IV Serum Replacements, 500 ml
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