产品英文名称：MycoAlert™ PLUS Mycoplasma Detection Kit

产品中文名称：MycoAlert™ PLUS 支原体检测试剂盒

MycoAlert™ PLUS支原体检测试剂盒　产品介绍：

MycoAlert™ PLUS Assay是一种精选生物化学检测方法，用于检测细胞培养过程中的支原体污染问题。支原体是一种能够自我复制的最小的原核微生物，和其他细菌不同的是它们没有细胞壁，所以细胞形态不固定，可以有多种变化，可以在培养基上形成极小的菌落。支原体是寄生生物，但同时也具有腐生功能。细胞培养过程中的污染支原体会使细胞生长变慢，影响细胞活性变化。新一代MycoAlert™ PLUS Assay可以检测出所有6种支原体属，多达180种支原体。与第一代MycoAlert™支原体检测试剂盒相比有更高的灵敏度和准确性。检测样品中的活性支原体裂解后，释放出的酶与MycoAlert™ PLUS Substrate反应，催化ADP转化为ATP。加入MycoAlert™ PLUS　Reagent试剂后在荧光素酶作用下ATP产生光信号(ATP+ Luciferin + O2 →Oxyluciferin + AMP + PPi + CO2 + LIGHT)。光信号可用光度计读出，根据第二次读数(Read B)与第一次读数(Read A)比率可是确定是否存在支原体污染。Read B/Read A比率<1, 支原体阴性，无支原体；1-1.2，不确定是否存在支原体，需重新检测；>1.2，支原体阳性，存在支原体污染。

产品优点：

» 生物发光技术，无需DNA提取物

» 样品检测只需两步移液操作，结果分析简单

» 可以和低灵敏性的板光度计或多功能读数器配用

» 可用于检测未启用培养基，培养基补充物或水溶液

» 快速方便，标准程序20分钟就可出结果

» 通用性强，可检测所有常见的支原体污染(不含脲原体属)

适合检测样品：

– 悬浮细胞上清液

– 未启用的培养基

– 10%-20% FCS or FBS

– L-谷氨酰胺

– 青霉素-链霉素混合物

– 中性胰蛋白酶(1:2稀释)

– 庆大霉素/两性霉素B

MycoAlert™ PLUS Mycoplasma Detection Kit:

Mycoplasma are the smallest and simplest prokaryotes. Mycoplasma depend on their hosts for

many nutrients due to their limited biosynthetic capabilities. They have long been recognized as

common contaminants of cells in continuous culture, but their presence may go undetected for months. As the mycoplasma competes with the cells for the nutrients in culture media, one of the first signs is a reduction in the rate of cell proliferation and slight changes in cellular responses, including gene expression.

Mycoplasma detection in cell cultures has, until now, been a long, drawn out process with difficult-tointerpret results. The MycoAlert™ PLUS Kit is intended for the quick and convenient detection of viable mycoplasma in cell cultures. The speed and convenience of the MycoAlert™ PLUS Kit allows for the routine testing of cells in culture and commonly used constituents of complete media.

To allow for the early detection of mycoplasma contamination, Lonza recommends testing at every cell passage. Frequent testing such as this will indicate when a cell line becomes infected, allowing prompt remedial action to be taken. The MycoAlert™ PLUS Assay can also be extended to incoming cell lines and the commonly used constituents of complete media.

订购信息：

• LT07-701 MycoAlert™ PLUS Mycoplasma Detection Kit, 10Tests

• LT07-703 MycoAlert™ PLUS Mycoplasma Detection Kit, 30Tests

• LT07-705 MycoAlert™ PLUS Mycoplasma Detection Kit, 50Tests

• LT07-710 MycoAlert™ PLUS Mycoplasma Detection Kit, 100Tests

• LT07-518 MycoAlert™ Assay Control Sets, 10Tests

• LT27-292 MycoAlert™ Assay Buffer, 20ml