产品英文名称：Mouse Hematopoietic Stem and ProgenitorCell Isolation Kit

产品中文名称：小鼠造血干细胞和祖细胞分离试剂盒

BD Stemflow™ Mouse Hematopoietic Stem and Progenitor Cell Isolation Kit的简介

试剂盒提供必要试剂借助多色流式细胞仪从小鼠骨髓样品分离造血祖细胞（HCPs）和造血干细胞（HSCs）。小鼠HCPs和HSCs具备一组细胞表面标志物，以及具有高水平的染料外排活性。BD小鼠造血干细胞和祖细胞分离试剂盒包含特异性标志物的荧光抗体和有助于识别HPCs和HSCs的荧光抗体。试剂盒中含有的特异性抗体包括：CD3的抗体（T细胞标志物），CD45R(B220)(B细胞标志物)，Ly6C和Ly6G(Gr1)(粒细胞标志物)，CD11b(Mac1)(巨噬细胞标志物)，以及TER-119(红细胞标志物)。试剂盒中同时含有3种特异性抗体用于分离HPCs和HSCs。第一个是CD34，它由HPCs表达，在干细胞中为阴性。另外两个是Sca-1和c-Kit(CD117)，它们由HPCs和HSCs表达。使用这些抗体去分离祖细胞和干细胞（称为CD34-/dim KSL细胞，KSL为c-Kit+/Sca-1+/Lineage-），这种分离方法已被广泛报道。每种特异性抗体都有非特异性同型对照。另外还含有7-AAD和BD CompBead 补偿颗粒。具体操作方法详见说明书。

Mouse Hematopoietic Stem and ProgenitorCell Isolation Kit 的物质组成

CD34 FITC, c-Kit PE, Sca-1 PE-Cy™7 and Lineage Cocktail APC; isotype controls; 7-AAD; BD™ CompBead compensation particles.

BD Stemflow™ Mouse Hematopoietic Stem and Progenitor Cell Isolation Kit的保存条件

The entire BD Mouse Hematopoietic Stem and Progenitor Cell Isolation Kit must be stored in the dark at 2–8°C. Do not freeze.

BD Stemflow™小鼠造血干细胞和祖细胞分离试剂盒的特征

1.Enables high purity isolation of HPC and HSC subpopulations from mouse bone marrow

2.Delivers a streamlined solution for consistentexperiments with fluorochrome-conjugated antibodies,compensation beads, protocols, and analysis guidelines

3.Facilitates compensation and scatter setup and conserves

cells with BD CompBead microparticles

4.Provides flexibility to drop in additional fluorochromeconjugated antibodies. Compatible with side population staining and upfront magnetic bead enrichment.

BD Stemflow™小鼠造血干细胞和祖细胞分离试剂盒的特别注意事项

The reagents in this kit contain sodium azide. Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.