产品英文名称：SensoLyte® 520 HCV Protease Assay Kit *Fluorimetric*

产品中文名称：SensoLyte® 520 HCV 蛋白酶荧光检测试剂盒

SensoLyte® 520 HCV Protease Assay Kit *Fluorimetric* 产品介绍

丙型肝炎病毒（HCV）NS3／4A蛋白酶是病毒在NS3-NS4A，ns4a-ns4b，ns4b-ns5a，和ns5a-ns5b这些位点非结构蛋白裂解所需的。这些裂解的病毒蛋白的成熟至关重要。因此，该蛋白酶成为开发抗HCV药物的关键目标之一。该 SensoLyte®520 HCV蛋白酶检测试剂盒提供了方便的测定。HCV NS3/4A蛋白酶抑制剂和连续定量高通量筛选HCV NS3/4A蛋白酶活性采用5-FAM / QXL™520.荧光共振能量转移（FRET）肽。这个肽序列是来自裂解位点NS4A / NS4B。在这个肽中，5-FAM荧光淬灭由QXL™520完成。在分裂成两个独立的片段的HCV NS3/4A蛋白酶（方案1），在荧光5-FAM回收，可在激发/发射= 490 nm / 520nm被监测。具有优异的荧光量子产率和更长的波长，5-FAM信号不受细胞成分和测试化合物的荧光。测定检测到低至1.56毫微克/毫升活跃的HCV NS3/4A蛋白酶。

SensoLyte® 520 HCV蛋白酶荧光检测试剂盒的成分

Component A HCV NS3/4A protease substrate 120 μL

Ex/Em=490 nm/520 nm upon cleavage

Component B 5-FAM, fluorescence reference standard 100 μM, 5 μL

Ex/Em=490 nm/520 nm upon cleavage

Component C 2X Assay buffer10 mL

Component D Stop solution10 mL

Component E DTT 1 M, 0.5 mL

Component F Pep4AK 50μL, 600μM

SensoLyte® 520 HCV Protease Assay Kit *Fluorimetric* 的保存

• Store all kit components at -20°C

• Protect Components A and B from light

• Components C and D can be stored at room temperature for convenience

SensoLyte® 520 HCV 蛋白酶荧光检测试剂盒的英文描述

The SensoLyte® 520 HCV Assay Kit uses a new FRET peptide substrate that incorporates 5-FAM (fluorophore) and QXL™ 520 (quencher) for a continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL™ 520. Upon cleavage of the FRET peptide by HCV NS3/4a protease, the fluorescence of 5-FAM is recovered and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, this 5-FAM/QXL™ 520-based FRET peptide shows less interference from autofluorescence of test compounds and cellular components. This assay is ten times more sensitive than an EDANS/DABCYL based assay and can detect 0.1 pmole of HCV NS3/4a protease. The assays are performed in a convenient 96-well or 384-well microplate format. The kit contains: • 5-FAM/QXL™ 520-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage) • Fluorescence reference standard for calibration • A detailed protocol.

SensoLyte® 520 HCV Protease Assay Kit *Fluorimetric* 系列产品订购信息

AnaSpec 71145 SensoLyte® 520 HCV Protease Assay Kit *Fluorimetric* 1 kit

AnaSpec 72168-5 HCV NS3/4A protease genotype 3a, recombinant 5 µg

AnaSpec 72166-5 HCV NS3/4A protease genotype 1a, recombinant 5 µg

AnaSpec 61017-10 HCV NS3/4A protease genotype 1b, recombinant 10 µg

AnaSpec 72168-10 HCV NS3/4A protease genotype 3a, recombinant 10 µg

AnaSpec 61017-5 HCV NS3/4A protease genotype 1b, recombinant 5 µg

AnaSpec 72166-10 HCV NS3/4A protease genotype 1a, recombinant 10 µg

AnaSpec 72169-10 HCV NS3/4A protease genotype 4d, recombinant 10 µg

AnaSpec 72167-10 HCV NS3/4A protease genotype 2a, recombinant 10 µg

AnaSpec 72167-5 HCV NS3/4A protease genotype 2a, recombinant 5 µg

AnaSpec 72169-5 HCV NS3/4A protease genotype 4d, recombinant 5 µg