产品英文名称：SensoLyte® 620 HCV Protease Assay Kit *Fluorimetric*

产品中文名称：SensoLyte®620 HCV 蛋白酶荧光检测试剂盒

SensoLyte® 620 HCV Protease Assay Kit *Fluorimetric* 产品介绍

丙型肝炎病毒（HCV）NS3／4A蛋白酶是病毒在NS3-NS4A，ns4a-ns4b，ns4b-ns5a，和ns5a-ns5b这些位点非结构蛋白裂解所需的。这些裂解的病毒蛋白的成熟至关重要。因此，该蛋白酶成为开发抗HCV药物的关键目标之一。该SensoLyte® 620 HCV蛋白酶检测试剂盒提供了高流率的一个方便的测定。HCV NS3/4A蛋白酶抑制剂和HCV NS3/4A连续定量筛选使用Hilyte氟蛋白酶活性TR/QXL™610荧光共振能量转移（FRET）肽。这个肽序列是来自裂解位点NS4A / NS4B。在苦恼的肽，HiLyte Fluor™r荧光淬灭通过QXL™610来完成。在分裂成两个独立的片段的HCV NS3/4A蛋白酶（方案1），HiLyte Fluor™TR是可以回收的，可监测激发/发射= 591 nm和622 nm。与附近的红色波长，Hilyte Fluor™TR信号避免大多数测试化合物的干扰，因此是理想的流率HCV NS3/4A蛋白酶抑制剂的筛选。

SensoLyte® 620 HCV Protease Assay Kit *Fluorimetric* 的组分

Component A HCV NS3/4A protease substrate 120 μL

Ex/Em=591 nm/622 nm upon cleavage

Component B HiLyte Fluor™TR, fluorescence reference standard 100 μM, 5 μL

Ex/Em=591 nm/622 nm

Component C 2X Assay buffer 10 mL

Component D Stop solution 10 mL

Component E DTT 1 M, 0.5 mL

Component F Pep4AK 50 μL, 600 μM

HCV NS3 protease cofactor

SensoLyte® 620 HCV Protease Assay Kit *Fluorimetric* 的保存

• Store all kit components at -20°C

• Protect Components A and B from light

• Components C and D can be stored at room temperature for convenience

SensoLyte® 620 HCV 蛋白酶荧光检测试剂盒的英文描述

The SensoLyte® 620 HCV Assay Kit uses a new FRET peptide substrate that incorporates HiLyte Fluor™ TR (fluorophore) and QXL™ 610 (quencher) for a continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of Hilyte Fluor™ TR is quenched by QXL™ 610. Upon cleavage of the FRET peptide by HCV NS3/4a protease, the fluorescence of Hilyte Fluor™ TR is recovered and can be continuously monitored at excitation/emission = 590 nm/620 nm. With near red emission wavelength, this HiLyte Fluor™ TR/QXL™ 610-based FRET peptide avoids the autofluorescence interference from most test compounds. This feature makes this FRET peptide an ideal substrate for screening HCV protease inhibitors since some of screening compounds have strong autofluorescence at shorter wavelength, making other FRET peptide substrates inappropriate. The assays are performed in a convenient 96-well or 384-well microplate format.

The kit contains:

• HiLyte Fluor™ TR/QXL™ 610-based FRET peptide substrate (Ex/Em=590/620 nm upon cleavage)

• Assay buffer

• Fluorescence reference standard for calibration

• A detailed protocol

Kit Size: 100 assays

SensoLyte® 620 HCV Protease Assay Kit *Fluorimetric* 系列产品订购信息

AnaSpec 71146 SensoLyte® 620 HCV Protease Assay Kit *Fluorimetric* 1 kit

AnaSpec 72169-5 HCV NS3/4A protease genotype 4d, recombinant 5 µg

AnaSpec 72168-5 HCV NS3/4A protease genotype 3a, recombinant 5 µg

AnaSpec 72166-5 HCV NS3/4A protease genotype 1a, recombinant 5 µg

AnaSpec 61017-10 HCV NS3/4A protease genotype 1b, recombinant 10 µg

AnaSpec 72168-10 HCV NS3/4A protease genotype 3a, recombinant 10 µg

AnaSpec 61017-5 HCV NS3/4A protease genotype 1b, recombinant 5 µg

AnaSpec 72166-10 HCV NS3/4A protease genotype 1a, recombinant 10 µg

AnaSpec 72169-10 HCV NS3/4A protease genotype 4d, recombinant 10 µg

AnaSpec 72167-10 HCV NS3/4A protease genotype 2a, recombinant 10 µg

AnaSpec 72167-5 HCV NS3/4A protease genotype 2a, recombinant 5 µg