产品英文名称: RoboSep™ Human Whole Blood Lymphoid Positive Selection Kit with Filter Tips

产品中文名称: RoboSep™ 带有过滤嘴的人全血淋巴阳性选择试剂盒

RoboSep™ Human Whole Blood Lymphoid Positive Selection Kit with Filter Tips

产品英文描述

RoboSep™ Human Whole Blood Lymphoid Positive Selection Kit with Filter Tips is designed to isolate CD3+ and CD19+ cells from whole blood by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD3, CD19 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD3 antigen is expressed on T lymphocytes and NKT cells while the CD19 antigen is expressed on B cells and follicular dendritic cells.

RoboSep™ Human Whole Blood Lymphoid Positive Selection Kit with Filter Tips

Recommended for

The isolation of human CD3+ and CD19+ cells from whole blood by positive selection

RoboSep™ 带有过滤嘴的人全血淋巴阳性选择试剂盒产品组成

• EasySep™ Human Whole Blood Lymphoid Positive Selection Cocktail, 3 x 1.0 mL

• EasySep™ Magnetic Particles, 3 x 1.0 mL

• EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL

RoboSep™ 带有过滤嘴的人全血淋巴阳性选择试剂盒保存方法

POSITIVE SELECTION COCKTAIL

Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 – 25°C) and should be refrigerated upon receipt.

EASYSEP® MAGNETIC NANOPARTICLES

Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 – 25°C) and should be refrigerated upon receipt.

EASYSEP® RBC LYSIS BUFFER 10X CONCENTRATE

10X concentrate stable at room temperature (15 - 25°C) until expiry date as indicated on label. Store at room temperature (15 - 25°C). 1X Lysis Buffer is stable at 2 - 8°C for 3 months. Store at 2 - 8°C. Do not freeze.

RoboSep™ 带有过滤嘴的人全血淋巴阳性选择试剂盒实验图

FACS Histogram Results Using EasySep® Human Whole Blood Lymphoid Positive Selection Kit

Starting with fresh whole blood, the CD2+/CD20+ cell content of the selected fraction typically ranges from 97.9% - 99.9%. *Red blood cells were removed by lysis prior to flow cytometry.

RoboSep™ Human Whole Blood Lymphoid Positive Selection Kit with Filter Tips

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For research use only. Not intended for human or animal diagnostic or therapeutic uses.