英文名称：Recombinant Human Fibroblast Growth Factor-basic

中文简称：【FGF 2】重组人碱性成纤维细胞生长因子

Recombinant Human Fibroblast Growth Factor-basic 【FGF 2】重组人碱性成纤维细胞生长因子 产品描述：

碱性成纤维细胞生长因子（basic fibroblast growth factor，bFGF）是成纤维细胞生长因子蛋白（fibroblast growth factors，FGFs）家族中的一员。目前已发现至少22种FGF，其中N3F-1（aFGF）、FGF-2（bFGF）及FGF-7的研究较为深入。bFGF是哺乳动物和人体中一种非常微量的活性物质，具有广泛的生理功能和重要的临床应用价值。bFGF不仅能刺激新生血管形成，参与创伤愈合和组织再生，促进胚胎组织发育和分化等，此外还与肿瘤的发生发展密切相关。近年来，重组bFGF已被应用于临床治疗创伤、溃疡及神经系统等疾病。

人碱性成纤维细胞生长因子重组蛋白是在大肠杆菌中重组表达的，是一条单链、非糖基化的多肽链，包含有155个氨基酸，分子量为17353Da。该人碱性成纤维细胞生长因子重组蛋白最终由标准的色谱技术纯化出。

来源宿主---大肠杆菌中重组表达。

Recombinant Human Fibroblast Growth Factor-basic 【FGF 2】重组人碱性成纤维细胞生长因子产品实验须知：

溶解：提供的人碱性成纤维细胞生长因子(FGF 2)产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含1mg/ml人碱性成纤维细胞生长因子(FGF 2)的PBS（pH 7.4）溶液中冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100ug/ml，后继实验过程中可根据实验要求进一步稀释。

保存：人碱性成纤维细胞生长因子(FGF 2)冻干粉末在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。

Recombinant Human Fibroblast Growth Factor-basic 【FGF 2】重组人碱性成纤维细胞生长因子 产品英文描述：

Synonyms

Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.

Introduction

Basic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.

Description

Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17353 Dalton.

The FGF-b is purified by proprietary chromatographic techniques.

Source (来源)

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized from a concentrated (1mg/ml) solution in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized Fibroblast Growth Factor Basic in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability （稳定性)

Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-b should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity （纯度）

Greater than 96.0% as determined by(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE.

Amino acid sequence （氨基酸序列）

MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS.

Biological Activity (生物活性）

The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5 ng/ml, corresponding to a specific activity of 2,000,000 Units/mg.

Protein content （蛋白质含量）

Protein quantitation was carried out by two independent methods

1. UV spectroscopy at 280 nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of Fibroblast Growth Factor-b as a Reference Standard.