英文名称：Recombinant Mouse Granulocyte Macrophage-Colony Stimulating Factor

中文简称：【mGM CSF 】重组小鼠粒-巨噬细胞集落刺激因子

Recombinant Mouse Granulocyte Macrophage-Colony Stimulating Factor 【mGM CSF 】重组小鼠粒-巨噬细胞集落刺激因子 描述：

粒-巨噬细胞集落刺激因子（granulocyte-macrophage colony-stimulating factor,GM-CSF）为造血生长因子家族成员，通过细胞膜表面特异性受体（GM-CSF receptor,GMR）介导维持细胞存活，刺激细胞增殖与分化,，调节成熟细胞功能。人和小鼠GM-CSF分别由144和141氨基酸残基组成，均包含17氨基酸的先导序列。成熟的人和小鼠GM-CSF分子分别由127和124个氨基酸残基组成，在氨基酸水平上有54%同源性，但生物学作用具有种属特异性。

小鼠粒-巨噬细胞集落刺激因子重组蛋白是在大肠杆菌中重组表达的，是一条单链、非糖基化的多肽链，包含有125个氨基酸，分子量为14285.35Da。该小鼠粒-巨噬细胞集落刺激因子重组蛋白最终由标准的色谱技术纯化出。

来源宿主---大肠杆菌中重组表达

Recombinant Mouse Granulocyte Macrophage-Colony Stimulating Factor 【mGM CSF 】重组小鼠粒-巨噬细胞集落刺激因子使用须知：

溶解：提供的小鼠粒-巨噬细胞集落刺激因子（mGM CSF）产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含小鼠粒-巨噬细胞集落刺激因子（mGM CSF）的溶液（不含添加剂）中冻干产生的。我们建议加无菌的20mM AcOH (乙酸)以配制工作储存液到100ug/ml，后继实验过程中可根据实验要求进一步稀释。

保存：小鼠粒-巨噬细胞集落刺激因子 (mGM CSF )冻干粉末在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。

Recombinant Mouse Granulocyte Macrophage-Colony Stimulating Factor 【mGM CSF 】重组小鼠粒-巨噬细胞集落刺激因子 英文描述：

Synonyms

CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim.

Introduction

GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.

GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Description

Granulocyte Macrophage Colony Stimulating Factor Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14285.35 Dalton.

GM-CSF Mouse is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

GM-CSF Mouse was lyophilized with no additives.

Solubility（溶解）

It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 20mM AcOH (acetic Acid) not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability （稳定性）

Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GM-CSF should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity （纯度）

Greater than 98.0% as determined by

(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE.

Amino acid sequence （氨基酸序列）

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Arg.

Biological Activity （生物活性）

The ED50 as determined by the dose-dependant stimulation of the proliferation of murine FDC-P1 cell line is < 0.2 ng/ml, corresponding to a Specific Activity of 5,000,000 IU/mg.

Protein content （蛋白质含量）

GM-CSF quantitation was carried out by two independent methods

1. UV spectroscopy at 280 nm using the absorbency value of 0.765 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of GM-CSF as a Reference Standard.