英文名称：Recombinant Human Tumor Necrosis Factor-beta

中文简称：【TNF b】重组人肿瘤坏死因子β

Recombinant Human Tumor Necrosis Factor-beta 【TNF b】重组人肿瘤坏死因子β 细胞因子产品描述：

淋巴毒素alpha,肿瘤坏死因子家族的一员,是一个淋巴细胞产生的细胞因子。LTA高度诱导、分泌和homotrimeric分子存在。LTA形式与lymphotoxin-beta heterotrimers锚lymphotoxin-alpha到细胞表面。LTA介导多种炎症、免疫刺激性和抗病毒反应。本公司也在开发过程中参与二级淋巴器官的形成,在细胞凋亡过程中发挥作用。

人肿瘤坏死因子β重组蛋白是在大肠杆菌中重组表达的，是一条单链、非糖基化的多肽链，包含有172个氨基酸，分子量为18645Da。该人肿瘤坏死因子β重组蛋白最终由标准的色谱技术纯化出。

来源宿主---大肠杆菌中重组表达

Recombinant Human Tumor Necrosis Factor-beta 【TNF b】重组人肿瘤坏死因子β 细胞因子产品使用须知：

溶解：提供的人肿瘤坏死因子β(TNF b)产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含人肿瘤坏死因子β(TNF b)的溶液（不含添加剂）中冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到不低于100ug/ml，后继实验过程中可根据实验要求进一步稀释。

保存：人肿瘤坏死因子β(TNF b)在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。

Recombinant Human Tumor Necrosis Factor-beta 【TNF b】重组人肿瘤坏死因子β 细胞因子产品英文描述：

Synonyms

Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, LTA, LT, TNFB, TNFSF1.

Introduction

Lymphotoxin alpha, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. LTA is highly inducible, secreted, and exists as homotrimeric molecule. LTA forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface. LTA mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. LTA is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.

Description

Tumor Necrosis Factor-b Human Recombinant (Lymphotoxin) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 172 amino acids and having a molecular mass of 18645 Dalton.

The TNF-b is purified by standard chromatographic techniques.

Source （来源）

Escherichia Coli.

Physical Appearance （外观）

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized protein with no additives.

Solubility （溶解性）

It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-beta in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability （稳定性）

Lyophilized Tumor Necrosis Factor-b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-b should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity （纯度）

Greater than 98.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAG.

Amino acid sequence （氨基酸序列）

MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL.

Biological Activity （生物活性）

The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is < 0.05ng/ml, corresponding to a Specific Activity of 20,000,000IU/mg.

Protein （蛋白质含量）

content Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 1.082 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of TNF-b as a Reference Standard.

Usage Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.