英文名称：Recombinant Mouse Tumor Necrosis Factor-alpha

中文简称：【mTNF a】重组小鼠肿瘤坏死因子α

Recombinant Mouse Tumor Necrosis Factor-alpha 【mTNF a】重组小鼠肿瘤坏死因子α 细胞因子产品描述：

肿瘤坏死因子-α(Tumor Necrosis Factor-alpha，TNF-α)是一种主要由单核-吞噬细胞产生的单核因子，不仅具有选择性地杀伤某些肿瘤细胞，而且有多种免疫调节作用。生理条件下具有生物学活性的人肿瘤坏死因子α（hTNF-α）是一个紧密的三聚体，其活性位点位于两个相邻亚基之间的V型结构域，hTNF-α有两种不同的受体，分别介导不同的生物学活性，在对hTNF-α结构与功能研究的基础上，人们对其N末端、C末端和p75受体结合位点进行基因改造。研制了一系列衍生物，以提高hTNF-α的抗肿瘤活性，降低毒副反应。

小鼠肿瘤坏死因子α重组蛋白是在变铅青链霉菌中重组表达的，是一条单链、非糖基化的多肽链，包含有156个氨基酸，分子量为17301.32Da。该小鼠肿瘤坏死因子α重组蛋白最终由标准的色谱技术纯化出。

来源宿主---变铅青链霉菌中重组表达

Recombinant Mouse Tumor Necrosis Factor-alpha 【mTNF a】重组小鼠肿瘤坏死因子α 细胞因子产品使用须知：

溶解：提供的小鼠肿瘤坏死因子α(mTNF a)产品为过滤灭菌的白色冻干粉末；该冻干粉末中每毫克小鼠肿瘤坏死因子α是从50mM Tris-HCL（pH 8），20mM NaCL溶液中冻干产生的。 我们建议加无菌的18MΩ-cm水以配制工作储存液到不低于100ug/ml，后继实验过程中可根据实验要求进一步稀释。

保存：小鼠肿瘤坏死因子α(mTNF a)在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。

Recombinant Mouse Tumor Necrosis Factor-alpha 【mTNF a】重组小鼠肿瘤坏死因子α 细胞因子产品英文描述：

Synonyms

TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.

Introduction

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.

TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells.

Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.

Description

Tumor Necrosis Factor-a Mouse Recombinant produced in E. coli is a single, non glycosylated, polypeptide chain containing 157 amino acids and having a molecular mass of 17301.32 Dalton.

The TNF-alpha is purified by standard chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance （外观）

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.2.

Solubility （溶解性）

It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability （稳定性）

Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity （纯度）

Greater than 97.0% as determined by:

(a) Analysis by RP-HPLC.

(c) Analysis by SDS-PAGE.

Amino acid sequence （氨基酸序列）

MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL

Biological Activity （生物活性）

The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is < 0.1ng/ml, corresponding to a Specific Activity of 10,000,000 Units/mg.

Protein content （蛋白质含量）

Protein quantitation was carried out by two independent methods

1. UV spectroscopy at 280 nm using the absorbency value of 1.24 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of TNF-a as a Reference Standard.