英文名称：Recombinant Human Insulin Like Growth Factor-2

中文简称：【IGF2】重组人胰岛素样生长因子-2

Recombinant Human Insulin Like Growth Factor-2 【IGF2】重组人胰岛素样生长因子-2 细胞因子产品描述：

胰岛素样生长因子2(IGF2)又称为生长调节素A(somatomedin A)，是目前所知功能最复杂多样的生长因子。刚转录出来的原始IGF2是由156个氨基酸残基组成的多肽链，经过后续的剪切加工，成熟IGF2是一类由67个氨基酸残基组成的小分子弱酸性多肽，分子质量7 471 u(不过在人的脊髓液中还发现一种9 ku的IGF2，在人脑中发现了更高分子质量的IGF2，在人血清发现了一种15 ku的IGF2)。IGF2能促进细胞的有丝分裂和分化，参与生后基因组重建，与X染色体的失活有重要关系。所有的这些功能说明IGF2在机体生长发育过程中的重要性。此外，IGF2与个体生长速度、瘦肉率、背膘厚等生产性能关系密切，在某些物种已经被作为候选基因确定下来。作为重要的印记基因，IGF2的表达状况与肿瘤，以及潜在肿瘤的发生发展关系密切，在肿瘤治疗方面起重要作用。

人胰岛素样生长因子-2重组蛋白是在大肠杆菌中重组表达的，是一条单链、非糖基化的多肽链，包含有67个氨基酸，分子量为7505Da。该人胰岛素样生长因子-2重组蛋白最终由标准的色谱技术纯化出。

来源宿主---大肠杆菌中重组表达

Recombinant Human Insulin Like Growth Factor-2 【IGF2】重组人胰岛素样生长因子-2 细胞因子产品产品须知：

溶解：提供的人胰岛素样生长因子-2(IGF2)产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含人胰岛素样生长因子-2(IGF2)的溶液（不含添加剂）中冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100ug/ml，后继实验过程中可根据实验要求进一步稀释。

保存：人胰岛素样生长因子-2(IGF2)冻干粉末在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。

Synonyms

Somatomedin-A, IGF2, INSIGF, pp9974, C11orf43, FLJ22066, FLJ44734.

Introduction

IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.

Description

Insulin-Like Growth Factor-II Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 67 amino acids and having a molecular mass of 7505 Dalton.

IGF-II is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance （外观）

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized with no additives.

Solubility （溶解度）

It is recommended to reconstitute the lyophilized IGF2 in sterile 18MΩ-cm H2O at a concentration of 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability （稳定性）

Lyophilized IGF-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFII should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity （纯度）

Greater than 97.0% as determined by

(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE.

Amino acid sequence （氨基酸序列）

AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE.

Biological Activity （生物活性）

The ED50, calculated by the dose-dependent induction of MCF-7 cell proliferation was found to be 0.85ng/ml corresponding to a Specific Activity of 1,176,470IU/mg.

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.