英文名称：Recombinant Mouse Interleukin-4

中文简称：【mIL 4】重组小鼠白介素-4

Recombinant Mouse Interleukin-4 【mIL 4】重组小鼠白介素-4 细胞因子产品描述：

Interleukin-4是主要由产生的多效细胞激活T淋巴细胞,嗜碱性粒细胞和肥大细胞。多种免疫response-modulating函数是由IL-4各种细胞类型的调节器,它有一个重要的角色同形像切换、诱导B淋巴细胞和分化的IgE辅助T细胞。IL-4绑定由细胞膜和分泌可溶性IL-4受体。

小鼠白介素-4重组蛋白是在大肠杆菌中重组表达的，是一条单链、非糖基化的多肽链，包含有120个氨基酸，分子量为13500Da。该小鼠白介素-4重组蛋白最终由标准的色谱技术纯化出。

来源宿主---大肠杆菌中重组表达

Recombinant Mouse Interleukin-4 【mIL 4】重组小鼠白介素-4 细胞因子产品使用须知：

溶解：提供的小鼠白介素-4(mIL 4)产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含1mg/ml小鼠白介素-4(mIL 4)的溶液（不含添加剂）中冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100ug/ml，后继实验过程中可根据实验要求进一步稀释。

保存：小鼠白介素-4(mIL 4)冻干粉末在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。

Recombinant Mouse Interleukin-4 【mIL 4】重组小鼠白介素-4 细胞因子产品英文描述：

Synonyms

BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.

Introduction

Interleukin-4 is a pleiotropic cytokine produced primarily by activated T lymphocytes, basophils and mast cells. Multiple immune response-modulating functions are performed by IL-4 on a variety of cell types and it has an important role in the regulator of isotype switching, induction of IgE production in B lymphocytes and differentiation of precursor T helper cells. IL-4 binds to both membrane-bound and secreted soluble IL-4 receptors.

Description

Interleukin-4 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 120 amino acids and having a molecular mass of 13500 Dalton.

The IL-4 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance （外观）

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2µm concentrated (1mg/ml) solution in PBS pH 7.4.

Solubility （溶解性）

It is recommended to reconstitute the lyophilized Interleukin 4 in sterile 10mM HAc not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability （稳定性）

Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL4 should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity （纯度）

Greater than 96.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE.

Amino acid sequence （氨基酸序列）

MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S.

Biological Activity （生物活性）

The ED50 as determined by the dose-dependant induction of HT-2 cell proliferation is less than 2 ng/ml corresponding to a Specific Activity of 500,000IU/mg.