英文名称：Recombinant Mouse Fibroblast Growth Factor-basic

中文简称：【mFGF 2】重组小鼠碱性成纤维细胞生长因子

Recombinant Mouse Fibroblast Growth Factor-basic 【mFGF 2】重组小鼠碱性成纤维细胞生长因子产品描述：

碱性成纤维细胞生长因子（basic fibroblast growth factor，bFGF）是成纤维细胞生长因子蛋白（fibroblast growth factors，FGFs）家族中的一员。目前已发现至少22种FGF，其中N3F-1（aFGF）、FGF-2（bFGF）及FGF-7的研究较为深入。bFGF是哺乳动物和人体中一种非常微量的活性物质，具有广泛的生理功能和重要的临床应用价值。bFGF不仅能刺激新生血管形成，参与创伤愈合和组织再生，促进胚胎组织发育和分化等，此外还与肿瘤的发生发展密切相关。近年来，重组bFGF已被应用于临床治疗创伤、溃疡及神经系统等疾病。

小鼠碱性成纤维细胞生长因子重组蛋白是在大肠杆菌中重组表达的，是一条单链、非糖基化的多肽链，包含有145个氨基酸，分子量为16320Da。该小鼠碱性成纤维细胞生长因子重组蛋白最终由标准的色谱技术纯化出。

来源宿主---大肠杆菌中重组表达

Recombinant Mouse Fibroblast Growth Factor-basic 【mFGF 2】重组小鼠碱性成纤维细胞生长因子产品使用须知：

溶解：提供的小鼠碱性成纤维细胞生长因子 (mFGF 2)产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含小鼠碱性成纤维细胞生长因子 (mFGF 2)的5mM NaP（pH 7.5）和50mM NaCl溶液中冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100ug/ml，后继实验过程中可根据实验要求进一步稀释。

保存：小鼠碱性成纤维细胞生长因子 (mFGF 2)冻干粉末在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。

Recombinant Mouse Fibroblast Growth Factor-basic 【mFGF 2】重组小鼠碱性成纤维细胞生长因子产品英文描述：

Synonyms

HBGH-2, HBGF-2, Prostatropin, FGF-2, FGB-b.

Introduction

FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. there are differences in the tissue distribution and concentration of these 2 growth factors.

Description

Fibroblast Growth Factor-basic Mouse Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16.3kDa. The FGF-2 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance （物理特征）

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

FGF-b was lyophilized from 5mM Na2PO4, pH7.5 and 50mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized Fibroblast Growth Factor b in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability （稳定性）

Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-basic should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity （纯度）

Greater than 95.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE.

Amino acid sequence （氨基酸序列）

MPALPEDGGA AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS.

Biological Activity （生物活性）

The activity as calculated by the dose-dependant proliferation of BALB/3T3 cells was found to be 0.1-0.16ng/ml.

Protein content （蛋白质含量）

Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.885 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of FGF2 as a Reference Standard.