代理Roche 罗氏 DIG DNA 地高辛标记DNA和检测试剂盒 Labeling and Detection Kit 现货


产品英文名称:DIG DNA Labeling and Detection Kit

产品中文名称:地高辛标记DNA和检测试剂盒

罗氏 DIG DNA 地高辛标记DNA和检测试剂盒的应用:

Random-primed labeling of DNA probes with DIG-11-dUTP, alkali-labile and color detection of DIG-labeled hybrids. DIG-labeled probes are used for hybridization in Southern, northern, and dot blots, as well as for colony and plaque screening.

DIG-labeled hybrids are detected with an anti-DIG-alkaline phosphatase conjugate and the substrates NBT (nitroblue tetrazolium salt) and BCIP (5-bromo-4-chloro-3-indolyl phosphate, toluidinium salt), which give a light-blue precipitate.

Roche DIG DNA Labeling and Detection Kit 组分:

Unlabeled Control DNA 1, 100 µg/ml

2. Unlabeled Control DNA 2, 200 µg/ml

3. DNA Dilution Buffer

4. DIG-labeled Control DNA, 5.2 µg/ml

5. 10x Hexanucleotide Mix

6. 10x dNTP Labeling Mixture

7. Klenow Enzyme, Labeling grade, 2 U/µl

8. Anti-digoxigenin-AP-conjugate, 750 U/ml

9. NBT/BCIP Concentrated Stock Solution

10. Blocking Reagent

Roche 罗氏 DIG DNA 地高辛标记DNA和检测试剂盒 Labeling and Detection Kit 检测原理:

高效DNA地高辛标记检测试剂盒I是用地高辛(Digoxigenin,简称DIG;又称异羟基洋地黄毒甙元,是一种类固醇半抗原分子)标记的DNA探针,应用于分子杂交和随后的显色反应检测。罗氏公司的该试剂盒检测主要分三阶段进行:1)DNA标记:根据随机引物标记技术,生成DIG地高辛标记的DNA探针。2)杂交:按照标准杂交方法进行,将地高辛标记的DNA探针用于核酸点杂交,可选择带正电的尼龙膜或纯硝酸纤维素膜作为杂交膜。 3)免疫学检测:首先将标记有AP的地高辛抗体与杂交探针免疫结合;通过检测碱性磷酸酶与NBT/BCIP底物进行显色反应的数值,从而达到免疫检测的目的。

Roche 罗氏 DIG DNA 地高辛标记DNA和检测试剂盒 Labeling and Detection Kit 实验图像:

罗氏 DIG DNA 地高辛标记DNA和检测试剂盒的产品描述:

Sensitivity and specificity: The standard labeling reaction with 1 µg template will give a yield of 260 ng DIG-labeled DNA after a 1-hour incubation at +37°C, or 780 ng after a 20-hour incubation. The sensitivity of DIG detection depends on the concentration of the DIG-labeled probe in the hybridization reaction, and the duration of the color reaction.

Table 1: Sensitivity of DIG detection.

© 金山科研平台 Thermo Fisher官网Nunc、QSP、Nalgene、Invitrogen、Gibco耗材试剂授权一级代理是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:https://thermonunc.cn/thread-19750.htm
产品询价需求提交
返回