产品英文名称：DIG DNA Labeling and Detection Kit

产品中文名称：地高辛标记DNA和检测试剂盒

罗氏 DIG DNA 地高辛标记DNA和检测试剂盒的应用：

Random-primed labeling of DNA probes with DIG-11-dUTP, alkali-labile and color detection of DIG-labeled hybrids. DIG-labeled probes are used for hybridization in Southern, northern, and dot blots, as well as for colony and plaque screening.

DIG-labeled hybrids are detected with an anti-DIG-alkaline phosphatase conjugate and the substrates NBT (nitroblue tetrazolium salt) and BCIP (5-bromo-4-chloro-3-indolyl phosphate, toluidinium salt), which give a light-blue precipitate.

Roche DIG DNA Labeling and Detection Kit 组分：

Unlabeled Control DNA 1, 100 µg/ml

2. Unlabeled Control DNA 2, 200 µg/ml

3. DNA Dilution Buffer

4. DIG-labeled Control DNA, 5.2 µg/ml

5. 10x Hexanucleotide Mix

6. 10x dNTP Labeling Mixture

7. Klenow Enzyme, Labeling grade, 2 U/µl

8. Anti-digoxigenin-AP-conjugate, 750 U/ml

9. NBT/BCIP Concentrated Stock Solution

10. Blocking Reagent

Roche 罗氏 DIG DNA 地高辛标记DNA和检测试剂盒 Labeling and Detection Kit 检测原理:

高效DNA地高辛标记检测试剂盒I是用地高辛（Digoxigenin，简称DIG；又称异羟基洋地黄毒甙元，是一种类固醇半抗原分子）标记的DNA探针，应用于分子杂交和随后的显色反应检测。罗氏公司的该试剂盒检测主要分三阶段进行：1）DNA标记：根据随机引物标记技术，生成DIG地高辛标记的DNA探针。2）杂交：按照标准杂交方法进行，将地高辛标记的DNA探针用于核酸点杂交，可选择带正电的尼龙膜或纯硝酸纤维素膜作为杂交膜。 3）免疫学检测：首先将标记有AP的地高辛抗体与杂交探针免疫结合；通过检测碱性磷酸酶与NBT/BCIP底物进行显色反应的数值，从而达到免疫检测的目的。

Roche 罗氏 DIG DNA 地高辛标记DNA和检测试剂盒 Labeling and Detection Kit 实验图像：

罗氏 DIG DNA 地高辛标记DNA和检测试剂盒的产品描述：

Sensitivity and specificity: The standard labeling reaction with 1 µg template will give a yield of 260 ng DIG-labeled DNA after a 1-hour incubation at +37°C, or 780 ng after a 20-hour incubation. The sensitivity of DIG detection depends on the concentration of the DIG-labeled probe in the hybridization reaction, and the duration of the color reaction.

Table 1: Sensitivity of DIG detection.