产品英文名称：Ficoll PM400

产品中文名称：聚蔗糖PM400

GE 聚蔗糖PM400（干粉）Ficoll PM400简介：

高分子量的蔗糖聚合物由蔗糖与表氯醇共聚而成。该分子是高度支化的和羟基的高含量导致在含水介质中的溶解性非常好。

为了生产密度梯度的细胞和亚细胞成分的离心或沉降在单位重力分离。

蔗糖的中性的，高度支化的，亲水性聚合物，该聚合物容易溶解在水溶液中。

高达50％的浓度（重量/体积），覆盖多达1.2克/毫升的密度，就可以实现。

更好的渗透性能比蔗糖。

保留功能和形态的完整性。

可用于分离细胞，离心敏感和（下沉降在单位重力条件下）分离密度相近，但不同大小的细胞。

不穿透生物膜。

作为原料制备的菲可 - 帕克梯度。

用在其它的应用，如电泳，杂交，冷冻保存，并作为半抗原的载体。

Ficoll PM400是一种合成的中性的，高度支化的亲水性的蔗糖为400000的平均分子量的聚合物，它一直被用来形成密度梯度分离和隔离真核细胞，细胞器和细菌细胞中，作为稳定剂，并作为分离单核细胞制剂媒体。应用程序也可以在限定的培养基，核酸杂交，电泳和免疫学研究发现。

GE 聚蔗糖PM400（干粉）Ficoll PM400英文描述：

Application： Used in the formation of density gradients for the separation and isolation of eukaryotic cells, organelles, and bacterial cells, as a stabilizing agent and as a separation medium for the isolation of lymphocytes.

It also has applications in defined culture media, nucleic acid hybridization, electrophoresis, and immunological studies.

Used for gradient centrifugation in all types of centrifuge rotors and for separation at unit gravity.

Density Max.：1.2 g/ml

Endotoxin Activity Max.：0.12 EU/mg

Estimated Shelf Life from Manufacture Date：5 years

Storage Conditions ：4 - 30°C

Stability：Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Physical State：Solid

Physical Form：Spray-dried powder

Molecular Weight [Mr] Max.：~ 5 x 10~ 5

Molecular Weight [Mr] Min.：~ 3 x 105

Reactivity and Stability：The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevated temperatures. In neutral solutions, however, Ficoll PM 400 can be sterilized by autoclaving at 110°C for 30 min without degradation. Strong oxidizing and reducing agents should be avoided.

Stokes Radius：~ 10 nm

Color：White

Specific Rotation [alpha]20：50°-65°

Intrinsic Viscosity ：~ 17 ml/g

Solubility ：It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be attained