产品英文名称：ITS Liquid Media Supplement (100×)

产品中文名称：细胞培养添加因子混合物（合剂）

Sigma 细胞培养添加因子混合物（合剂）ITS Liquid Media Supplement (100×) I3146-5mL产品英文描述：

Quality Level GMP

sterility sterile-filtered

product line BioReagent

form liquid

impurities endotoxin, tested

suitability suitable for cell culture

storage temp. 2～8°C

Components

Contains 1.0 mg/ml bovine insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite.

Other Notes

ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.

Preparation Note

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Application

Serum-free and reduced serum formulations often require purified forms of the factors normally supplied by serum that are suitable for in vitro growth and differentiation studies. These important factors include insulin, transferrin, selenium, pyruvate, and ethanolamine.

Nutritional studies indicate that the supplement components described are utilized by most mammalian cells. They enhance cell proliferation and decrease the serum requirement of many cell types. When the provided supplement is used with 2 to 4 percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum.

Sigma 细胞培养添加因子混合物（合剂）ITS Liquid Media Supplement (100×) I3146-5mL应用：

无血清和降低血清制剂通常需要的是适合于在体外生长和分化的研究通常由血清供给的因素纯化形式。这些重要因素，包括胰岛素，转铁蛋白，硒，丙酮酸盐和乙醇胺。

Sigma 细胞培养添加因子混合物（合剂）ITS Liquid Media Supplement (100×) I3146-5mL使用注意事项：

ITS是重组人胰岛素，人转铁蛋白，亚硒酸钠的混合物。它是一个普通的细胞补充设计用于在非复合培养基使用（例如，MEM，RPMI-1640）和复合培养基（e.g. Ham?F-12，DME/ F-12，MEM）与丙酮酸钠。

无酚红制备Earle氏平衡盐溶液（EBSS）。