产品英文名称：LEAF™ Purified anti-human CD3

产品中文名称：LEAF™纯化的抗人CD3抗体

BioLegend LEAF™ CD3抗体 纯化，抗人英文描述：

Clone: UCHT1

Isotype: Mouse IgG1, κ

Isotype Control:LEAF™ Purified Mouse IgG1, κ Isotype Ctrl

Workshop

Number: III 471

Reactivity: Human, Cross-Reactivity: Chimpanzee

Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.

Preparation: The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration: 1.0 mg/ml

Storage & Handling: The CD3 antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.

Application:

FC - Quality tested

CyTOF® - Validated

IP, Activ, IHC, WB - Reported in the literature

BioLegend LEAF™ CD3抗体 纯化，抗人推荐用法：

每一批这种抗体是免疫荧光染色与流式细胞仪检测的质量控制。流式细胞仪染色，建议使用这种试剂是≤2.0每百万微克细胞在100μl体积或100微升全血。因此建议该试剂滴定为每个应用程序的最佳性能。

BioLegend LEAF™ CD3抗体 纯化，抗人应用注意事项：

附加的报告应用程序（有关格式）包括：丙酮固定的冰冻sections4,6,7和福尔马林固定的石蜡包埋的sections11，immunoprecipitation1，活化了T cells2,3,5，和西blotting9免疫组织化学染色。叶™纯化抗体（内毒素<0.1欧/微克，叠氮化物，无0.2微米过滤）被推荐用于功能分析（目录300414号）。对于高度敏感的分析，我们建议超LEAF™纯化抗体（货号300438），具有较低的内毒素限值比标准LEAF™纯化抗体（内毒素<0.01 EU /微克）。

BioLegend LEAF™ CD3抗体 纯化，抗人实验图像：

Human peripheral blood lymphocytes stained with LEAF™ purified UCHT1 and then detected with anti-mouse IgGs FITC

Human peripheral blood mononuclear cells were stained with CFSE on day 0, and then stimulated with (filled histogram) or without (open histogram) immobilized LEAF™ Purified CD3 (clone UCHT1) and LEAF™ purified CD28 (clone CD28.2) for 3 days. On day 4, cells were harvested and stained with CD4 Brilliant Violet 711™. Dot plot (above) was analyzed on live cells. Histogram data (below) was analyzed by gating on CD4 positive cells (above).

BioLegend LEAF™ CD3抗体 纯化，抗人相关产品订购信息：

300413 50 µg

300414 500 µg

300432 1 mg