产品货号：ZZK-K-XYLS

产品品名：木聚糖酶AX检测试剂盒

英文品名：Xylanase (Xylazyme AX) Assay Kit

规格型号：200 assays per kit

For the measurement of endo-1,4-?-D-xylanase in enzyme preparations,bread improver mixtures and animal feeds. Contains Xylazyme AX Tabletsand xylanase enzyme controls (A. niger and Trichoderma longibrachiatum).

INTRODUCTION:

Arabinoxylan is the major endosperm cell-wall polysaccharide of

wheat and rye and is found in significant proportions in most cereal

solutions and slurries of high viscosity, and in animal nutrition it

reduces the rate of nutrient absorption from the gut.

endo-Beta-D-Xylanase (xylanase) is added to feeds to catalyse

depolymerisation of this polysaccharide. It can be demonstrated

that endo-cleavage by xylanase of just one bond per thousand in the

arabinoxylan backbone can significantly remove viscosity properties.

Of the carbohydrase enzymes used as feed supplements, one of the

most difficult to measure has been xylanase. These problems are

attributed to several factors, including the low levels of enzyme

added to the feed, inactivation of enzyme during pelleting, binding

of the enzyme to feed components and the presence of specific

xylanase inhibitors.

The only biochemical methods which are sufficiently sensitive, specific

and robust to measure xylanase in feeds are viscometric assays and

those employing dyed xylan or arabinoxylan polysaccharides.

Viscometric assays are tedious, whereas assays employing dyed

xylan substrates are rapid, reproducible and simple to perform.

We recommend the use of either Xylazyme AX tablets or Azo-

Wheat Arabinoxylan (Azo-WAX). Xylazyme AX based assays

are about 5-fold more sensitive than assays employing Azo-WAX.

However, this latter substrate does have sufficient sensitivity in most

applications, and results are slightly more reproducible than with

Xylazyme AX.

It is generally accepted that xylanase enzymes which are best suited

to feed applications have optimal activity at pH 6.0. Consequently,

these enzymes are generally assayed at this pH in 100 mM sodium

phosphate buffer. In recovery experiments, however, we found that

sodium phosphate buffer extracts only a small proportion (< 20%)

of the amount of enzyme added to the feed. Thus a wide range of

alternative extractants and extraction conditions have been evaluated.

For feeds containing Trichoderma sp. xylanases, the best and most

consistent results have been obtained using 100 mM acetic acid

or 100 mM sodium acetate buffer (pH 4.7) at room temperature.

Optimal extraction of Humicola sp. xylanases was achieved with a

buffer containing 100 mM MES buffer (pH 6.0) and 1 % w/v sodium

dodecyl sulphate (SDS).

KITS:

Kits containing the required reagents to measure xylanase in animal

feeds are available from Megazyme. These kits contain:

1. Xylazyme AX test tablets (200 tablets).

2. A. niger control xylanase (~ 295 mU/mL at 40°C and pH 4.7)

in 50 % (v/v) glycerol (activity stated on vial).

3. T. longibrachiatum control xylanase (~ 386 mU/mL at 40°C and

pH 6.0) in 50% (v/v) glycerol (activity stated on vial).

爱尔兰Megazyme公司是全球知名的酶及酶法分析试剂盒的生产厂家，

其中大多数产品都被AOAC(Association of Official Analytical Chemists)、

AACC(American Association of Cereal Chemists)、ICC(InternationalAssociation for Cereal Science and Technology)、

EBC(European Brewing Convention)、RACI(Royal Australian Chemical Institute)等国际组织采用。

其中欧洲检测果汁及葡萄酒等发酵产品基本都采用该公司产 品。

其中全线产品及相关检测信息见：

该公司产品分以下系列：

1. 生物及食品酶法分析试剂盒 (Diagnostic Kits)

2. 试剂混合物(Reagent Mixtures)

3. 协同因子(Cofactors)

4. 糖酶片剂试验(Carbohydrase Tablet Tests)

5. 蛋白酶片剂试验(Protease Test Tablets)

6. 可溶性呈色底物(Soluble Chromogenic Substrates)

7. 不可溶性（交联的）呈色底物(Insoluble (Crosslinked) Chromogenic Substrates)

8. 酶(Enzymes)

9. 植物凝血素(Lectins)

10. 多聚糖(Polysaccharides)

11. 低聚糖(Oligosaccharides)

12. 相关产品 (General)

其中产品被国际机构采用，建立国际检测方法如下：

总淀粉检测盒（Total Starch Assay Kit）

AOAC Method 996.11

AACC Method 76-13

ICC Standard Method no. 168

RACI Standard Method

总膳食纤维检测盒（Total Dietary Fibre Assay Kit）

AOAC Method 985.29

AOAC Method 991.42

AOAC Method 993.19

AOAC Method 991.43

AACC Method 32.07

AACC Method 32.21

AACC Method 32.05

淀粉损失检测盒（Starch Damage Assay Kit）

AACC Method 76-31

ICC Standard No. 164

RACI Standard Method

α-淀FEN酶检测盒Ceralpha (α-Amylase) Assay Kit

AOAC Method 2002.01

ICC Standard No. 303

RACI Standard Method

CCFRA Flour Testing Working Group Method 0018

AACC – under evaluation.

Alpha淀FEN酶检测盒Amylazyme (α-Amylase Assay)

AACC Method 22.05

RACI Standard Method

果聚糖检测盒Fructan Assay Kit

AOAC Method 999.03

AACC Method 32-32

木聚糖酶AX片剂（Xylazyme AX Tablets）

Xylazyme AX Tablets have been widely adopted in the fermentation andfeeds industries for the measurement of Xylanase.

Beta-葡聚糖检测盒Beta-Glucan (Mixed Linkage) Assay Kit

AACC Method 32-23

EBC Methods 3.11.1, 4.16.1 and 8.11.1

AOAC Method 995.16

ICC Standard No. 166

RACI Standard Method

极限糊精酶和支链淀FEN酶检测片剂（Limit-Dextrizyme）

RACI Standard Method

Beta-葡聚糖酶检测盒（Beta-Glucanase (Malt and Microbial) Assay Kit）

RACI Standard Method

Beta-淀FEN酶检测盒（Betamyl (Beta-Amylase Assay Kit)）

RACI Standard Method.

木聚糖酶检测底物（Azo-Wheat Arabinoxylan ）

Azo-Wheat Arabinoxylan, Azo-CM-Cellulose and Ceralpha methods for theassay of Xylanase, cellulase and ?-Amylase, respectively, have beenadopted by the UK silage industry.

抗性淀粉检测盒（Resistant Starch Assay Kit）

AOAC Method 2002.02

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