品牌：Peprotech

规格：10µg

别名:

soluble Receptor Activator of NF-κB Ligand, TNFSF11, TRANCE (TNF-related activation-induced cytokine), OPGL, ODF (Osteoclast differentiation factor)

描述:

RANKL and RANK are members of the TNF superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone

turnover. RANK (receptor) was originally identified as a dendritic cell-membrane protein, which, by interacting with RANKL, augments the ability of dendritic cells.

These dendritic cells then stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, promote the survival of RANK + T-cells, and regulate

T-cell-dependent immune response. RANKL, which is expressed in a variety of cells, including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal

cells, is also capable of interacting with a decoy receptor called OPG. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis

by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage.

Recombinant Murine sRANKL is a 19.4 kDa polypeptide comprising the TNF-homologous region of RANKL (174 amino acid residues).

氨基酸序列:

PAMMEGSWLD VAQRGKPEAQ PFAHLTINAA SIPSGSHKVT

LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR

HHETSGSVPT DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN

WSGNSEFHFY SINVGGFFKL RAGEEISIQV SNPSLLDPDQ

DATYFGAFKV QDID

来源: E.coli

纯度: Greater than 98% by SDS-PAGE gel and HPLC analyses.

生物活性: Determined by its ability to induce NF-κB in RAW264.7 cells in the absence of any cross-linking.

The expected ED50 for this effect is 10.0-25.0 ng/ml.

交叉反应: Human, Rat, Mouse

研究领域：