Catalog number: HRP-60008

Size: 50 μg/100 μl

Source: Mouse

Isotype: IgM

Synonyms: ACTB; ACTB, Acn, acn, beta,Acn, cytoplasmic 1, B acn,Beta acn, F acn, HRP β acn,HRP-ACTB, HRP-Beta acn,HRP-F acn, PS1TP5BP1, βacn

Applications:

Tested applicaons: ELISA, WB

Species specificity: Human, Mouse, Rat, Zebra Fish; other species nottested.

Caculated beta Actin MW: 375aa,42 kDa

Observedbeta ActinMW: 42 kDa

Posive WB detected in : HeLa cells

Recommended diluon: WB: 1:2000-1:20000

Background:

Beta acn, also named as ACTB and F-Acn, belongs to the acn family. Acns arehighly conserved globular proteins that are involved in various types of cell molityand are ubiquitously expressed in all eukaryoc cells. At least six isoforms of acnsare known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1),alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aorc smooth muscle), beta (ACTB),gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are twonon-muscle acn proteins. Most acns consist of 376aa, while ACTG2 (rich inmuscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta acnhas been widely used as the internal control in RT-PCR and Western Blo&ng as a 42-kDa protein. This anbody can recognize all of acns. This anbody is conjugatedwith HRP.