DSHB CD133 HB#7，CD133/ Prominin-1（concentrate），规格：0.1mL，保存：4°CDSHB-耗材-金山科研平台 Thermo Fisher官网Nunc、QSP、Nalgene、Invitrogen、Gibco耗材试剂授权一级代理

DSHB CD133 HB#7，CD133/ Prominin-1（concentrate），规格：0.1mL，保存：4°CDSHB

货品编号：DSHB.CD133 HB#7

品牌：DSHB

品名：CD133/ Prominin-1（concentrate）

规格：0.1 mL

保存：4°C

Antigen:CD133/ Prominin-1	Hybridoma Cells Available:Yes
Antigen Species:Human	Depositor:Swaminathan, S.K. / Panyam, J. / Ohlfest, J.R.
Antigen Sequence:	Depositors Institution:University of Minnesota
Antigen Molecular Weight:predicted molecular weight 97 kDa	Human Protein Atlas:
Predicted Species Reactivity:	Depositors Notes:This antibody recognizes both glycosylated and non-glycoslyated extracellular domains of CD133.
Gene:PROM1	Immunogen:Recombinant chimeric protein
Alternate Gene Names:AC133, CD133, CORD12, MCDR2, PROML1, RP41, STGD4	Immunogen Sequence:aa180-380 and aa612-765
Isotype:MIgG1	Alternate Antibody Name:HC7
Clonality:Monoclonal	Alternate Antigen Name:
Epitope Mapped:No	Myeloma Strain:SP 2/0
Epitope Location or Sequence:	Uniprot ID:O43490
Host Species:mouse	Entrez Gene ID:8842
Additional Characterization:	Antibody Registry ID:
Additional Information:Depending upon glycosylation, the apparent molecular weight on western blots will increase to ~ 120 kDa. This antibody recognizes CD133 is aldehyde fixed cells and tissues (see 25448068 ).
Positive Tested Species Reactivity:Human	Recommended Applications:FACS, Immunofluorescence, Immunohistochemistry, Western Blot

Storage and Handling Recommendations

Usage Recommendations

Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts:1) a volume of antibody stored at 4°C to be used within two weeks.2) the remaining product diluted with an equal volume of molecular grade glycerol and stored either at -20°C or -80°C.

While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry(IHC), immunofluorescence(IF) and staining is 2-5 μg/ml.For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 μg/ml).

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DSHB CD133 HB#7，CD133/ Prominin-1（concentrate），规格：0.1mL，保存：4°CDSHB

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