DSHB E7,Tubulin, beta(concentrate),规格:0.1mL,保存:4°CDSHB


货品编号:DSHB.E7

品牌:DSHB

品名:Tubulin, beta(concentrate)

规格:0.1 mL

保存:4°C

Antigen:Tubulin, betaHybridoma Cells Available:Yes
Antigen Species:ChylamydomonasDepositor:Klymkowsky, Michael
Antigen Sequence:Depositors Institution:University of Colorado, Boulder, CO
Antigen Molecular Weight:55 kDaHuman Protein Atlas:
Predicted Species Reactivity:Depositors Notes:Stains methanol fixed cells.
Gene:TubbImmunogen:Recombinant protein
Alternate Gene Names:TUBBImmunogen Sequence:* see additional information
Isotype:MIgG1Alternate Antibody Name:
Clonality:MonoclonalAlternate Antigen Name:
Epitope Mapped:NoMyeloma Strain:P3
Epitope Location or Sequence:Uniprot ID:P07437
Host Species:mouseEntrez Gene ID:203068
Additional Characterization:Antibody Registry ID:AB_2315513
Additional Information:E7 was originally generated by Drs. M. McCutcheon and S. Carroll from a mouse immunized with Drosophila fushi tarazu gene product. However, the antibody reacts specifically with beta-tubulin from a wide range of species (Chamydomonas to human).
Positive Tested Species Reactivity:Chlamydomonas, Drosophila, Flatworm, Human, Kangaroo Rat, Mouse, XenopusRecommended Applications:Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Storage and Handling RecommendationsUsage Recommendations
Although many cell products are maintained at 4°C for yearswithout loss of activity, shelf-life at 4°C is highly variable.To ensure retention of antibody activity, we recommend aliquotting the product into two parts:1) a volume of antibody stored at 4°C to be used within two weeks.2) the remaining product diluted with an equal volume of molecular grade glycerol and stored either at -20°C or -80°C.While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry(IHC),immunofluorescence(IF) and staining is 2-5 μg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 μg/ml).

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