品名：RecombinantMouseHGF

规格：25ug

保存：-20°C~ -70 °C

说明书下载：RecombinantMouseHGF

Purity：>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level： <0.10 EU per 1 μg of the protein by the LAL method.

Activity：Measured in a cell proliferation assay using mIMCD‑3 mouse epithelial cells. The ED50 for this effect is typically 10-30 ng/mL.

Measured by its ability to bind Recombinant Mouse HGF R/c-MET Fc Chimera (Catalog # 7065-ME) in a functional ELISA with an estimated KD <0.2 nM.

Source：Mouse myeloma cell line, NS0-derived Gln33-Arg495 (alpha) & Val496-Leu728 (beta)

Accession #：Q53WS5

N-terminal Sequence Analysis： No results obtained: Gln33 predicted (alpha) & Val496 (beta)

Predicted Molecular Mass：53.4 ( alpha -chain), 26 ( beta -chain) kDa

SDS-PAGE：60 kDa and 33 kDa, reducing conditions

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Formulation： Lyophilized from a 0.2 μm filtered solution in PBS.

Reconstitution：Reconstitute at 100 μg/mL in sterile PBS.

Shipping： The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.

1 month, 2 to 8 °C under sterile conditions after reconstitution.

3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HGF

HGF, also known as Scatter Factor and Hepatopoietin A, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1 - 5). Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha and 30 kDa beta chain. Alternate splicing generates an isoform that lacks the peptidase and the second, third, and fourth Kringle domains. Mouse HGF shares 91% - 95% aa sequence identity with bovine, canine, feline, human, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF‑dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up‑regulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin‑1 alpha ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).