货品编号： NEB.T1020S

品牌：NEB

品名： Monarch™ DNA Gel Extraction Kit

产品描述

The Monarch DNA Gel Extraction Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from

agarose gels. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure

zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers

provided have been optimized, anddo not require monitoring of pH. Unlike other kits, there is no need to add isopropanol

to the melted agarose prior to loading on the column, saving you a step. Eluted DNA is ready for use in restriction digests,

DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use

significantly less plastic and responsibly-sourced, recyclable packaging

Monarch DNA Cleanup Column Design Monarch columns are designed for performance

Monarch columns are designed without a frit, which eliminates buffer

retention and the risk of carryover contamination, providing fast, worry-free DNA purification. Advantages:

Elute in as little as 6 μl

Prevent buffer retention and salt carry-over with optimized column design

Save time with fast, user-friendly protocols

No need to monitor pH or add isopropanol

Buffers and columns available separately

Significantly less plastic used when compared with other kits

Responsibly-sourced and recyclable packaging

Specifications

Monarch DNA Gel Extraction Kit Protocol DNA purified from the Monarch DNA Gel Extraction Kit is recovered with similar efficiency and

purity as the leading supplier, but is more highly concentrated, facilitating its use in downstream applications One microgram aliquots of a 3 kb fragment were resolved on a 1% w/v agarose gel, excised, and processed with

different kits using manufacturer-specified minimum elution volumes. Values reported are the concentration and

purity data determined by Nanodrop™ readings, as well as recovery calculations based on the eluted DNA concentration and recovered volume. Monarch DNA Gel Extraction Kit reproducibly recovers DNA over a broad range of molecular weights.

A mixture of 7 DNA fragments ranging from 10 kb down to 0.5 kb was prepared and one-half of the

mixture was resolved on a 1% gel. Each fragment was manually excised from the agarose gel and

processed using the Monarch DNA Gel Extraction Kit. The entire elution of each fragment was resolved

on a new gel with the remainder of the original mixture for comparison. DNA purified from agarose gels using the Monarch DNA Gel Extraction Kit can be reproducibly isolated and ligated. Two micrograms of a 3 kb fragment with compatible ends was resolved on a 1% agarose gel, excised,

and purified using the Monarch DNA Gel Extraction Kit. Samples were eluted in 20 μl and a fraction (1/4 th of total)

was ligated using the Blunt/TA Ligase Master Mix . Representative samples from 5 replicates were

resolved on a second 1% agarose gel. M is the 1 kb DNA Ladder .