货品编号： signalchem.u01-11g-05

品牌： signalchem

品名： ULK1, Active

规格： 5 ug

Product Details

Description:Recombinant human ULK1 (1-649) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species:Human

Tag:GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence:1-649

Genbank Number:BC111603

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation

and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight : ~125 kDa

Gene Aliases : ATG1, UNC51, Unc51.1, FLJ38455

Scientific Background : ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital

response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein.

Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of

mTOR (1). ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy (2). Both FIP200 and

ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR

pathway in a nutrient starvation-regulated manner.

References : 1. Ganley I G, et al: ULK1.ATG13.FIP200 complex mediates mTOR signaling and is essential for autophagy. J Biol Chem.

2009 May 1;284(18):12297-305. 2. Chan E Y, et al: siRNA screening of the kinome identifies ULK1 as a multidomain modulator of autophagy. J Biol Chem. 2007 Aug 31;

282(35):25464-74.

Formulation :50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.