货品编号： Signalchem.B08-11BG-10

品牌： Signalchem

品名： BRAF, Active

规格： 10 ug

Product Details Description:Recombinant human BRAF (381-end or exon 9-18) protein was expressed by baculovirus in Sf9 insect cells

using an N-terminal GST tag. Species:Human Tag:GST tag Expression System:Sf9 insect cells using baculovirus Sequence:381-end or exon 9-18 Genbank Number:NM_004333 Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after

centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple

freeze/thaw cycles. Applications :Kinase Assay, Western Blot Molecular Weight :~69 kDa Gene Aliases :BRAF (Ex9-18), BRAF1, RAFB1, B-raf, B-raf 1, MGC126806, MGC138284 Formulation :50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. Scientific Background :BRAF is a member of the RAF family that is activated by members of the Ras family upon growth

factor-induced stimulation. Active Ras can induce heterodimerization of cRaf and BRAF and this may explain the observed

cooperativity of cRaf and BRAF in cells responding to growth factor signals (1). Activating mutations in the BRAF gene are present

in a large percentage of human malignant melanomas and in a proportion of colon cancers. The vast majority of these mutations result

in a valine to glutamic acid change at residue 599 within the activation segment of BRAF (2). References : 1. Weber, C K. et al: Active Ras induces heterodimerization of cRaf and BRaf. Cancer Res. 2001 May 1;61(9):3595-8. 2. Mercer, K E. et al: Raf proteins and cancer: B-Raf is identified as a mutational target. Biochim Biophys Acta. 2003 Jun 5;1653(1):25-40.