货品编号： Invitrogen.11995-040

品牌： Invitrogen

品名： DMEM, High Glucose, Pyruvate

规格： 1000 mL

储存条件： 2-8° C

产品描述

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different

mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and

smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of

DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows: With Without • High Glucose• HEPES • L-glutamine • Phenol Red • Sodium Pyruvate

The complete formulation is available.

Using DMEM DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original

Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is

often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors.

Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate

buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.