Catalog number: HRP-60004

Size: 75 μg/100 μl

Source: Mouse

Isotype: IgG2b

Synonyms:GAPDH; G3PD, GAPD, GAPDH,HRP-G3PD, HRP-GAPD, HRPGAPDH,HRP-OK/SW cl.12,OK/SW cl.12

Applications:

Tested applicaons: ELISA, WB

Cited applicaons: WB

Species specificity: Human, Mouse, Rat, Zebra Fish; other species nottested. Cited species: Human

Caculated GAPDH MW: 36 kDa

ObservedGAPDHMW: 36 kDa

Posive WB detected in : HeLa cells

Recommended diluon: WB: 1:5000-1:50000

Background:

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes thephosphorylaon of glyceraldehyde-3-phosphate during glycolysis. GAPDHparcipates in nuclear events including transcripon, binding RNA, RNAtransportaon, DNA replicaon, DNA repair and apoptosis. Being stably andconstuvely expressed at high levels in most ssues and cells, GAPDH is considereda housekeeping protein. It was widely used as a control for RT-PCR and also loadingcontrol in electrophoresis and Western blo'ng. GAPDH is normally expressed incellular cytoplasm or membrane, but can occasionally translocated to the nucleuspost modificaon such as S-nitrosylaon. This anbody is a mouse monoclonalanbody raised against full length GAPDH of human origin. It can recognize the36kda GAPDH protein in most cells/ssues. Please note that some physiologicalfactors, such as hypoxia and diabetes, increase GAPDH expression in certain celltypes. This anbody is conjugated with HRP.

Note: Do not add Azium (Sodium Azide or Smite) into the diluon buffer. Azium isthe HRP inhibitor which decreases the enzymac acvity of HRP.

Immunogen information:

Immunogen: Recombinant Protein

GenBank accession number: BC004109

Gene ID (NCBI): 2597

Full name: Glyceraldehyde-3-phosphate dehydrogenase

Product information

Purificaon method: Protein A Purified

Storage: PBS with 0.02% Thimerosal, 50% Glycerol, pH7.3. Store at -20oC.

Western blot analysis of GAPDH invarious ssues and cell lines usingProteintech anbody HRP-60004 at adiluon of 1:10000.