货品编号： Invitrogen.11960-069

品牌： Invitrogen

品名： DMEM, High Glucose, no Glutamine

规格： 10×500ml

产品描述

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different

mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth

muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range

of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With Without • High Glucose • L-glutamine • Phenol Red • Sodium Pyruvate • HEPES

The complete formulation is available.

Using DMEM DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's

Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with

higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM

requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L),

and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Product use For human ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal law restricts

this device to sale by or on the order of a physician. Customers using Gibco® DMEM in a manufacturing process, who have a submission

with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a

medical device manufacturer and is certified to ISO 13485 standards.