货品编号： Signalchem.M28-14G-50

品牌： Signalchem

品名： ERK2, Unactive

规格： 50 ug

Product Details Description: Recombinant full-length human ERK2 was expressed in E. coli cells using an N-terminal GST tag. Species: Human Tag: GST tag Expression System: E.coli Sequence: Full Length Genbank Number: NM_002745 Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after

centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple

freeze/thaw cycles. Applications : Kinase Assay, Western Blot Molecular Weight : ~68 kDa Gene Aliases : MAPK1, MAPK2, P42MAPK, PRKM1, PRKM2, p41mapk, ERK, ERT1 Scientific Background : ERK2 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases

(ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK2 requires both tyrosine

and threonine phosphorylation that is mediated by MEK. ERK2 is ubiquitously distributed in tissues with the highest expression in

heart, brain and spinal cord. Activated ERK2 translocates into the nucleus where it phosphorylates various transcription factors

(e.g., Elk-1, c-Myc, c-Jun, c-Fos, and C/EBP beta).

References : 1. Boulton, TG. et al: Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated

protein 2 kinase. Biochemistry. 1991 Jan 8;30(1):278-86. Formulation : 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.