货品编号： Invitrogen.L34964

品牌： Invitrogen

品名： LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit, for 405 nm excitation

规格： 400 assays

产品描述

The LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization

required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. This kit has been

optimized and validated for use with a violet laser flow cytometer. • Stable—dyes are freeze dried in separate vials to maintain stability • Robust—staining pattern is the same before and after fixation • Low compensation—minimal spectral overlap between other fluorophores View a selection guide for all fixable viability dyes for flow cytometry.

Stable Unlike products that are sold in solution, the LIVE/DEAD™ Fixable Violet Stain has been conveniently packaged in 40-test vials to help

ensure the stability and performance of the dye over time. Amine reactive dyes in solution will lose their effectiveness over a short period

of time, therefore it is recommended to completely use the vial once rehydrated. If this is not possible, aliquot the vials in small volumes

and store at -80°C, avoiding freeze-thaw cycles.

Robust Dead cell discriminator stains can lose sensitivity after treatment with fixatives such as formaldehyde or ethanol-based fixation methods

required for intracellular phosphorylation studies. The LIVE/DEAD™ Fixable Violet Stain is an amine reactive dye that binds covalently to

intracellular and extracellular amines, and the staining pattern is preserved following formaldehyde fixation.

Low compensation The LIVE/DEAD™ Fixable Violet Stain was selected based on its fluorescent properties to minimize compensation between other violet

dyes and dyes that excite off of the 488 nm blue laser. The violet-fluorescent reactive dye has an excitation maximum of ~416 nm and is

excited well with the 405 nm violet laser, and it has an emission maxima of ~451 nm, so it can be collected in the first channel on most

violet laser flow cytometers.

How it works In cells with compromised membranes, the dye reacts with free amines both in the cell interior and on the cell surface, yielding intense

fluorescent staining. In viable cells, the dye's reactivity is restricted to the cell-surface amines, resulting in less intense fluorescence. The

difference in intensity is typically greater than 50-fold between live and dead cells, allowing for easy discrimination.

Colors available LIVE/DEAD™ Fixable Dead Cell Stains are available in a wide variety of colors to meet your multi-color panel needs.