货品编号： Sigma.C-6885-25mg

品牌： Sigma

品名： II型胶原酶

规格： 25mg

Biochem/physiol Actions Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis

(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid

and 8-hydroxyquinoline. Collagenase is activated by four gram atom calcium, per mole enzyme. It is inhibited by ethylene glycol-bis

(β-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, β-mercaptoethanol, glutathione, thioglycolic acid and

8-hydroxyquinoline.Collagenase enzymes and neutral protease plays an important role in the effective release

of cells from tissue.[2] Collagenase recognizes the sequence -R-Pro-8-X-Gly-Pro-R-, where X is most often

a neutral amino acid.[4]

Caution As supplied, this product is stable for one year at -20°C. There is no loss in FALGPA or protease activity in 30

days at 37°C, 50°C and -20°C. Solutions of crude collagenase are stable if frozen quickly in aliquots (at 10 mg/mL)

and kept frozen at -20°C. Further freeze-thaw cycles will damage the solution. The product retains 100% activity

over 7 hours when held on ice.

包装 100, 500 mg in poly bottle

Preparation Note Also contains clostripain, nonspecific neutral protease, and tryptic activities. Unit Definition One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin

color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit

hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to

produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole

of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

Application Collagenase may be used: •for the preparation of arterial tissue for the study of advanced glycosylation end products (AGE) •for use along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium

and many other tissues. •in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes •for the preparation of viable hepatocytes from rat liver and for the isolation of fat cells from rat adipose tissue

General description Clostridium histolyticum is a pathogenic clostridium that produces collagenase. This is a mixture of enzymes containing

collagenase, non-specific proteases and clostripain.