货品编号： Cayman.520111

品牌： Cayman

品名： Leukotriene B4 ELISA Kit 96 strip wells

Features Measure LTB4 in plasma and other sample matrices Assay 24 samples in triplicate or 36 samples in duplicate Measure LTB4 levels down to 13 pg/ml Incubation: 18 hours | Development: 90-120 minutes | Read: Colorimetric at 405-420 nm

Synonyms LTB4 EIA Kit Leukotriene B4 (LTB4) is synthesized from arachidonic acid by the combined action of 5-lipoxygenase and

LTA4 hydrolase.1 LTB4 has long been recognized as a potent mediator of inflammation. It stimulates a number

of leukocyte functions, including aggregation, stimulation of ion fluxes, enhancement of lysosomal enzyme release,

superoxide anion production, chemotaxis, and chemokinesis.2,3 In subnanomolar ranges (3.9 x 10−10 M), LTB4

causes chemotaxis and chemokinesis in human PMNLs.2 At higher concentrations, (1.0 x 10−7 M), LTB4 leads to

neutrophil aggregation and degranulation as well as superoxide anion production.2,4 Plasma levels of LTB4 increase

from <100 pg/ml to >100 ng/ml following leukocyte stimulation.5,6,7 LTB4 is metabolized in leukocytes and hepatocytes

to less active 20-hydroxy and 20-carboxy LTB4 by NADPH-dependent cytochrome P450 enzymes followed by β-oxidation

at the ω-end to ω-carboxy dinor LTB4 and ω-carboxy tetranor LTB3.3,8,9,10 LTB4 is not excreted in the urine.11

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other

components are needed for this assay.