货品编号： Invitrogen.EF0651

品牌： Invitrogen

品名： FastAP Thermosensitive Alkaline Phosphatase

规格： 1000units

产品描述

Thermo Scientific FastAP Thermosensitive Alkaline Phosphatase catalyzes the release of 5'- and 3'-phosphate

groups from DNA, RNA, and nucleotides. This enzyme also removes phosphate groups from proteins.

FastAP is a novel alkaline phosphatase, which is active in all Thermo Scientific restriction enzyme buffers as well

as in PCR buffers. It dephosphorylates all types of DNA ends (blunt, 5'- and 3'-overhangs) in 10 minutes at 37°C.

The enzyme is inactivated in 5 minutes at 75°C (see Figure 1 in Supporting Data). Therefore, removal of alkaline

phosphatase is not required prior to ligation.

Highlights • Recombinant enzyme • Fast dephosphorylation—10 minutes at 37°C • Fast and complete inactivation—5 minutes at 75°C • Simultaneous digestion and dephosphorylation of vector DNA • 100% active in restriction enzyme and PCR buffers • PCR clean-up in conjunction with Exo I • Protein dephosphorylation

One protocol for all types of DNA ends: • 5'-overhangs • 3'-overhangs • blunt-ends • single nucleotides

Applications • Dephosphorylation of cloning vector DNA to prevent recircularization during ligation • Simultaneous digestion and dephosphorylation of vector DNA • PCR product clean-up: nucleotide degradation prior to sequencing of PCR product • Dephosphorylation of nucleic acid 5'-termini prior to labeling with T4 Polynucleotide Kinase • Other applications where dephosphorylation of DNA and RNA substrates is necessary • Protein dephosphorylation