Thermo 10372019 MCDB 131 Medium, no glutamine 500mlInvitrogen


货品编号: Thermo.10372019

品牌: Thermo

品名: MCDB 131 Medium, no glutamine

规格: 500ml

产品描述

MCDB 131 Medium was originally developed by Knedler and Ham as reduced serum-supplemented medium for

the culture of human microvascular endothelial cells (HMVEC). MCDB 131 Medium is also used with other cell

types, including human omental microvascular cells, hepatocytes, myocytes, and smooth muscle cells.

This MCDB 131 Medium is manufactured as follows:

With Without • Phenol Red • L-glutamine • Sodium pyruvate • HEPES

The complete formulation is available.

Using MCDB 131 MCDB 131 Medium contains many components not found in traditional basal media, such as trace elements, putrescine,

adenine, thymidine, and higher levels of some amino acids and vitamins. These additions allow the medium to be supplemented

with very low levels of serum or defined components. MCDB 131 Medium contains no proteins or growth factors, and is often

supplemented with EGF, hydrocortisone, glutamine, and low levels of Fetal Bovine Serum (FBS). The FBS concentration must

be optimized for each cell type. MCDB 131 Medium uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires

a 5–10% CO2 environment to maintain physiological pH.

Dual-site cGMP manufacturing For supply chain continuity, we manufacture MCDB 131 at two separate facilities, located in Grand Island, NY and Scotland, UK.

Both sites are compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as

medical device manufacturers.

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