货品编号： Sigma.S5761 品牌： Sigma 品名： 碳酸氢钠

规格： 1kg

Application Sodium bicarbonate has been used: •as a component of bovine embryo culture system during in vitro maturation, fertilization, and culture (IVMFC)[1] •as a component of a 1:1 mixture of Dulbecco′s modified Eagle′s medium and Ham′s nutrient mixture F-12 during

the culture of bovine microvascular endothelial cells[2] •as a component of ITS (insulin transferrin and selenite) medium used during the isolation of dopamine neurons

from human embryonic stem cells[3] •as a component of medium used for cell culture of human embryonic kidney cells[4] •as a component of Beltsville thawing solution used for the dilution of semen[5]

General description Sodium bicarbonate is broadly used as a physiological buffer in cell culture applications. The pKa for bicarbonate <->

carbonic acid reaction is 6.4 The pKa for bicarbonate <-> carbonate reaction is 10.3. Sodum bicarbonate has two pKas,

6.4 and 10.3. It is broadly used as an physiological buffer in in vitro applications.