货品编号: Invitrogen.IVGN2104 品牌: Invitrogen 品名: Anza T4 DNA Ligase Master Mix 规格: 50 reactions
产品描述:
The Invitrogen Anza™ T4 DNA Ligase Master Mix facilitates the joining of abutted 5’-phosphate and 3’-hydroxyl termini in duplex DNA
through the formation of a phosphodiester bond. It can be used to join DNA fragments with both sticky ends and blunt ends, and to repair
nicks in double-stranded DNA with 3'-hydroxyl and 5'-phosphate ends. Anza T4 DNA Ligase is formulated as a 4X concentrated master
mix. Ligation can be performed with DNA in water, TE, elution buffer, or 1X Anza™ buffers.
Benefits: • Ligation complete in 15 minutes at room temperature • A single ligase master mix for both sticky-end and blunt-end ligation reactions • Ready-to-use master mix format reduces pipetting steps • 4X concentration enables more dilute DNA to be used in ligation reaction without concentrating
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